the whole class. Each day will end with a keynote lecture by a prominent researcher in the field, putting the topic of the day into context through an example of relevant biological research. Contact: Julia Patricia Nohle; Email: julia.nohle@embl.de ...
coli (NEB) were used for proteins overexpression and DNA cloning, respectively. paTc-based vectors29 were used for tetracycline-inducible expression of CedA (paTc-cedA) and its truncated variant lacking first 11 amino acids (paTc-cedAΔ11N). Plasmids pVS10 30 and pSumoH10-cedA were used for ...
Since our retrogene discovery was limited to deletions corresponding to the full length of introns, a targeted discovery approach is likely to identify additional retrogenes present in the Dog10K collection, reinforcing retrogenes as an important class of canine genetic variation [72]....
Then, we asked whether NERD-seq can detect another important class of non-poly(A) ncRNAs: tRNAs, which are known and conserved RNA modification substrates. As shown in Fig.5A, NERD-seq produces a robust distribution around the TSS of these RNAs (see also Additional file 1: Fig. S7 for ...
ATP (NEB, 1 mM final) and protein kinase PLK3 (Thermo PV3812, 2 ng μl−1 final concentration) were added to the supernatant or pellet solutions, and the reactions were allowed to proceed for 24 h at 30 °C. The reactions were quenched and solubilized by the addition of...
cycle remains an important area for further investigation. The different localisation of the 3′UTR-derived fragments to their full-length counterparts raises more questions on the fate and function of these RNAs. It is worth mentioning that analysis of ribosome footprinting data [30] (not shown)...
(Sigma, MRN10). An NEB Next Ultra II RNA Library Prep Kit for Illumina (NEB, E7770S) was used to construct the library, and sequencing was performed on an Illumina NovaSeq6000 platform with 150-bp paired-end reads at Annoroad Gene Technology Co., Ltd. (Beijing, China). The clean ...
The secreted morphogen Dpp plays important roles in spatial regulation of gene expression and cell cycle progression in the developing Drosophila eye. Dpp signaling is required for timely cell cycle arrest ahead of the morphogenetic furrow as a prelude t
“first division restitution”. This has important genetic consequences, since it maintains parental heterozygosity around the centromeres, reducing the risk of deleterious alleles being exposed due to homozygotization [24,25,26]. The degree to which parental heterozygosity is restituted upon intra...
outliers;breads assigned to common soil invertebrate classes, with NA marking metazoan reads not assigned to soil invertebrates at the class level;csoil invertebrate richness trend along an elevation gradient (grey area marks standard error of the trendline). Assignments are available as Supplementary ...