For cell fixation, use 0.5 ml fixation buffer per tube and leave it in the dark for 20 minutes at room temperature. It is recommended that the reagent be titrated for optimal performance for each application. For the fixation procedure, please refer to the \"Intracellular Cytokine Staining Prot...
Biolegend抗体 420801 Fixation Buffer 品牌:Biolegend 品名:Fixation Buffer 货号:420801 规格:100 mL Clone:- 货期:现货 库存信息随时变更,准确货期以下单时确认为准。 Biolegend公司的试剂主要包括细胞免疫、细胞因子、趋化因子、粘附分子等及与癌症研究、T 调节细胞研究、干细胞研究、先天免疫研究、细胞周期分析、...
1️⃣ 细胞准备:以80%的密度铺板到6孔板,贴壁后加入LPS(推荐浓度1ug/mL),过夜培养。 2️⃣ 抗体染色:使用100 uL PBS重悬细胞,加入CD86和F4/80抗体,室温避光孵育30分钟。然后,用2 mL PBS洗涤细胞,离心收集。 3️⃣ 固定与破膜:用100 uL PBS重悬细胞,加入Fixation buffer固定细胞,室温孵育60分钟。...
RBC Lysis Buffer (10X)420301100 ml Fixation Buffer420801100 ml Intracellular Staining Permeabilization Wash Buffer (10X)421002100 ml Cell Staining Buffer420201500 ml FOXP3 Fix/Perm Buffer Set421403100 tests FITC anti-mouse CD410040550 ug APC/Cy7 anti-human HLA-DR307618100 tests ...
Biolegend常用产品表热销产品货号规格RBC Lysis Buffer (10X)420301100 mlFixation Buffer420801100 mlIntracellular Staining Permeabilization Wash Buffer
2017年11月24日 Biolegend Biolegend是世界著名流式抗体生产商,总部位于美国加州圣地亚哥,通过了 ISO 9001:2008 和 ISO 13485:2003 质量管理体系认证。Biolegend提供1万余种涉及细胞免疫分型、细胞因子和趋化因子、黏附因子、癌症研究、T辅助细胞、Tfh、干细胞、天然免疫、细胞周期分析、凋亡等多个热点研究领域的二十...
RBC Lysis Buffer (10X)420301100 ml Fixation Buffer420801100 ml Intracellular Staining Permeabilization Wash Buffer (10X)421002100 ml Cell Staining Buffer420201500 ml FOXP3 Fix/Perm Buffer Set421403100 tests FITC anti-mouse CD410040550 ug APC/Cy7 anti-human HLA-DR307618100 tests ...
FluoroFix Buffer FluoroFix(TM) Buffer is aready-to-use buffer, specially formulated for fixation of immunofluorescence stained cells, optimized to stabilize tandem dyes. It can be used as the final resuspension of the cell pellet in immunofluorescence staining procedures. FluoroFix(TM) Buffer is ...
货号 产品名称 规格 420301 RBC Lysis Buffer (10X) 100 ml 420801 Fixation Buffer 100 ml 421002 Intracellular Staining Permeabilization Wash Buffer (10X) 100 ml 420201 Cell Staining Buffer 500 ml 343522
420801 Fixation Buffer 650 100ml Biolegend 421002 Permeabilization Wash Buffer (10X) 1040 100ml Biolegend 421101 TMB Substrate Reagent Set 455 100ml Biolegend 421203 ELISA Assay Diluent (5X) 780 100ml Biolegend 421301 Propidium Iodide Solution 585 2ml Biolegend 421401 FOXP3 Fix/Perm Buffer (4x...