We discovered peculiar enzymes participating in selenium amino acid metabolism: selenocysteine β-lyase occurring in mammalian tissues and bacteria, and D-selenocystine α,β-lyase occurring in Clostridium sticklandii. We here describe enzymological properties and reaction mechanisms of ...
the UV light spectrum and peptide identification of purified products were same for three barches of preparation; K m and V max of recombinant α-galactosidase were 0.275 mmol/L and 0.014 mmol/min, respectively; optional temperature and pH of recombinant α-galactosidase reaction were 37℃ and ...
3b). K562 is the catalytic amino acid that plays a role in stabilizing the negative charge of transition state of the phosphoryl transfer reaction and protonate the leaving group. The spatial conservation of the catalytic site and good alignment with OLD_Bp may imply that GajA employs conserved ...
The biochemical basis for the reaction to canavanine-glycine-bromthymol blue (CGB) agar by Cryptococcus neoformans var. gattii and C. neoformans var. neoformans was investigated. All of the var. gattii isolates tested were found to utilize glycine as the sole source of carbon and nitrogen and ...
camphorataand cloned by polymerase chain reaction. The 1-Cys Prx cDNA (837bp, accession no. AY870325) contains an open reading frame encoding a protein of 223 amino acid residues with calculated molecular mass of 25,081Da. The deduced protein shared 44–58% identity with 1-Cys Prx fromHomo...
Physicochemical properties and emulsion stabilization of rice dreg glutelin conjugated with κ-carrageenan through Maillard reaction characterizationBACKGROUND: Rice dreg is an underutilized source of cereal protein with good potential for application in the food industry. Glutelin ... Yanxue,Du,Suhua,....
生化英文缩写（Biochemicalabbreviation）生化英文缩写（Biochemical abbreviation）Bio - chemical abbreviation Chapter 1 protein Amino acid classification 1. Non-polar fatty acids Gly glycine Ala alanine Val valine Leu leucine Ile isoleucine Pro proline 2. Polar neutral amino acids Ser serine Cys cysteine Me...
The genomic DNA for the two Drosophila genes, gstD1 and gstD21, were engineered for expression in Escherichia coli by polymerase chain reaction using a pair of specially designed primers. This newly designed expression system produced consistently high yields of the recombinant glutathione S-transfer...
Throughout the years, many methods have been used to accomplish this task, beginning with isoenzyme analyses and the use of repetitive DNA probes, to employing the polymerase chain reaction (PCR) and more state-of-the-art technologies. This review article summarizes the development of these ...
where k is the reaction rate constant [d− 1]. This also corresponds to the equation for the first CSTR reactor: (4)C1=Co1+k⋅V/Qgm−3 with C1 being the effluent concentration of the reactor [g m− 3]. The following applies to the second reactor, if its influent concentration...