IL-2 Bioassay Cells. When IL-2 binds to its receptor, receptor-mediated pathway signaling induces luminescence that can be detected upon addition of Bio-Glo™ Reagent and quantified with a luminometer. In the absence of IL-2, no signaling occurs downstream of IL-2R and no luminescent signal...
A highly sensitive and specific liquid chromatography-elctrospray ionization tandem mass spectrometry method was developed and validated for rapid determination of risperidone and its active metabolite in rat dried blood spots and dried plasma spots. Chromatographic separation was achieved through ZIC (R)...
There are two different types of signals that can be generated by a bioassay, a linear dose response and a sigmoidal (s-shaped) dose response. Either approach may be used. Sigmoidal bioassay designs are preferred if there is a clear saturation in the dose response and there is no compelling...
of TIGIT with its ligand, CD155. It is intended for use as a control with the PD-1+TIGIT Combination Bioassay and the TIGIT/CD155 Blockade Bioassay. These assays can be used to measure the potency and stability of antibodies and other biologics designed to block the TIGIT/CD155 interaction...
This is particularly evident in analysis of immune responses, which involve a variety of cell types. Currently, flow cytometry is the most useful technique for single cell analysis, due to its high-throughput and multiplexing capability. However, it cannot provide time-varying spatiotemporal ...
In its broader aspects therefore, the test reagent for the bioassay of von Willebrand's factor and platelet aggregating factor in human and animal blood plasmas comprises human or animal blood platelets, which may be freshly prepared, fixed, and/or air dried or lyophilized together with as a co...
For some time now the etiology has been confused with the diagnosis and the identification of the causative stimuli with the manifested effects. Although we don't know yet the entire story about the etiology of the oncogenic process we can say that we know a lot about its manifestations and ...
typesnotedabove,canbeassayedbymeasuringitsciliotoxicorciliarydyskinesiaryeffectonexplantpreparationsfromavarietyofciliatedtissues(2-6).551PergamonPres~552�CytolysisofColpidiumbyCysticFibrousSerum�Vol.15,No.3AlthoughthesebioassaysarereasonablyaccurateindetectingC/Ffactor,theirwidespreaduseislimitedbyseasonaland...
We provide evidence and necessary control experiments demonstrating that in planta, this ROS bioassay can be utilized to rapidly screen different Arabidopsis mutant lines and ecotypes in combination with different bacterial strains to investigate the genetic requirements of a plant host and its pathogen....
This is due to its superb sensitivity, easy to use and broad dynamic range. Coreactant-assisted ECLs of ruthenium complexes (including [Ru(bpy)3]2+) play substantial act in analytical and bioanalytical applications. Coreactant-assisted ECLs mainly consist of two types: I) oxidative–reductive ...