Enzyme, substrate, ATP, and compounds were diluted in a Kinase Buffer composed of 40 mM Tris (pH 7.5), 20 mM MgCl2, 0.1 mg/ml BSA, and 50 μM DTT. In a 384-well low-volume plate, 1 μl of the com
Enzyme, substrate, ATP, and compounds were diluted in a Kinase Buffer composed of 40 mM Tris (pH 7.5), 20 mM MgCl2, 0.1 mg/ml BSA, and 50 μM DTT. In a 384-well low-volume plate, 1 μl of the compound or 5% dimethyl sulfoxide (DMSO), 2 μl of PLK1 enzyme (...