For each ES protocol, we calculated the values for charge injected and accumulated and the effective field delivered. Subsequently, we explored how these ES regimes affect SH-SY5Y neuronal morphology, presynaptic marker expression, composition and concentration of secreted extracellular vesicles, as well...
Methods We registered the protocol for this review on PROSPERO, the international prospective register of systematic reviews of the University of York and the National Institute for Health Research, on June 11, 2021 (Huey et al. 2021). Inclusion and exclusion criteria Participants or models ...
Optimized Protocol for Protein Macrocomplexes Stabilization Using the EDC, 1-Ethyl-3-(3-(dimethylamino)propyl)carbodiimide, Zero-Length Cross-Linker 10/14/2014 Patents Cross-Linking Reagents for Molecular Interaction Analysis 07/16/2014 Publications ...
Additionally, RNA yields are more than sufficient to perform direct-RNA sequencing using Oxford Nanopore Technologies (ONT) platforms, which require 500 ng of total RNA per library (Manufacturer’s protocol, ONT kit SQK-RNA002). This enables the study of RNA modification changes during ...
(asthma) according to the ICD-10 classification. Planned admissions related to desensitisation, rehabilitation, and treatment according to the protocol of the drug programme were extracted from the collected data. Consequently, only hospitalisations related to the onset of asthma or COPD symptoms were...
The different processes includes organizing and preparing the desired biomass wastes; transport protocol; means to generate the energy required, and the specific operational techniques to be applied, which in most case are usually costly, painstaking, and time-consuming (Aziz, Hanafiah, & Gheewala, ...
Aptamers are single-strandednucleic acid(DNA, RNA) or peptide molecules with molecular weight less than 25kDa. Aptamer against any target/analyte can be selected in vitro throughSystematic evolution of ligands by exponential enrichmentprotocol from a large library of sequences (1015). Aptamers are ...
We also investigated the culture protocol to ensure that the constructs were fully mature even at the internal site. Methods ADSCs were isolated from three rats and expanded to create cartilage spheroids. The spheroids were arranged into patches using a Kenzan bio-3D printer to create scaffold-...
{RT}}\)) describes the temperature dependence of ionic conductivity in polymer electrolyte, where\(\sigma\)is ionic conductivity,\({\sigma }_{0}\)is pre-exponential factor,\({E}_{a}\)is activation energy,\(R\)is universal gas constant, and\(T\)is temperature [124]. Therefore, ...
mFAT inks were formulated by adapting a previously described protocol by Schüler et al.36. First, a 2% (w/w) soy protein isolate (SPI) suspension in ultrapure water was prepared, magnetically agitated at 50 °C for 5 h, and left overnight at 6 °C. Ultrapure water was then ad...