2ˆ 0.85% KCl-washed 2 mL 50 µL centrifuged, resuspended in ultrapure H2O 26 g 26 g washed with PBS cell lysis 2ˆ 20 s @ 5.0 bead beater (MP Biome-dicals, Lysing Matrix E Tubes) 40 s @ 6.0 bead beater (MP Bio-medicals, Lysing Matrix E Tubes) disruptor (Scientif. industr...