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Then, the proteins on gel were transferred to PVDF membranes. The membranes were blocked with 5% milk for 1 h at room temperature and followed by an overnight incubation with the primary anti-Aβ monoclonal antibody 6E10 (1:2000, Biolegend) at 4 °C overnight. Then, the membranes were ...
Then the darkness parted and I could see the daemon’s essence again, hovering over the body of the chirurgeon, my bowels turned to water. “It’s here.” I scramble away and she turns, draws her blade and deflects three kunai thrown at her from the three mistwraiths floating over ...