ECM的主要组分包括水、碳水化合物聚合物(如蛋白聚糖-Proteoglycan和透明质酸-Hyaluronic acid),以及蛋白质(如胶原蛋白-Collagen、弹性蛋白-elastin、纤维连接蛋白-fibronectin和层粘蛋白-laminin)。这些组分在ECM中各自扮演着重要角色,例如,蛋白聚糖负责缓冲、水合作用、结合和ECM的耐力特性,而胶原蛋白和纤维连接蛋白对于拉伸...
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Nanofibre assembly could be achieved for a wide range of ECM proteins including fibronectin, collagen and elastin. We could also apply this new process to prepare nanofibres from polysaccharides like hyaluronan and chitosan. Using scanning electron microscopy (SEM) we found that extruded nanofibres ...
Collagen (type I), fibrin, and Matrigel are the most prominently applied matrix materials and can be found in all three fields of application due to their excellent biological properties13,21,55,56. Interestingly, in muscle implants as well as meat blending of the protein-based matrix materials...
(Fig.3A). Muscle tissue turned white and translucent after decellularization. H&E and Masson’s trichrome staining indicated the retention of ECM, muscle fibers, and a small amount of collagen (Fig.3B). Following decellularization, DAPI staining and corresponding statistics showed that 97.9 ...
Collagen, fibrin and decellularized adipose tissue (DAT) were used as ECM for soft materials bio-ink. Human mesenchymal stem cells (hMSCs), SMCs, HeLa, hepatocarcinoma (HepG2), fibroblasts, ovary cells, keratinocytes, neural cells, BMSCs, chondrocytes, epithelial cells, ADSC, ovary cells, hepa...
ECM: Extracellular matrix GelMA: Gelatin methacryloyl hASCs: Human adipose stem cells HD: High-definition IBSC: Image-based single cell isolation IF: Impact factor iPSC: Induced pluripotent stem cell ISS: International space station PCL: Polycaprolactone PLA: Polylactic acid PLGA: Poly...
3.1. Collagen Collagen is a common mammalian protein and an important part of ECM. Their triple helix structure is similar to that of collagen fibrils in terms of chemistry, with each fibril being a polymer made up of repeated amino acids linked together by peptides. The most prevalent collage...
For use as scaffolds, many types of natural and synthetics polymers were investigated. The scaffolds used in tissue repairing are bone, tendon, cartilage, skeletal muscle, skin, ligament, vascular tissues, and neural tissues. Natural polymers, which are comprised of proteins (such as collagen, sil...
水凝胶是通过交联连接在一起的3D网络结构,具有高度亲水性和固有的生物相容性,是3D细胞培养和3D生物打印等组织工程应用的理想选择。来源于胶原蛋白、明胶和透明质酸的天然水凝胶具有很高的生物相容性,这是支持细胞活力、增殖、分化和运动的内在优势。这些独特的组成和结构与天然细胞外基质(ECM)相似,可以为细胞功能、粘附...