您是否使用电子翻译? [translate] afinding an enzyme inhibitor is the result of a good match of the biological and chemical spaces represented by a binding site of an enzyme and a 发现酶抑制剂是生物和化工空间的一次好比赛的结果酵素和a的束缚位置代表的 [translate] ...
From a conceptual viewpoint, several distinct modes of RNA–enzyme interaction can be envisaged (Figure 4, Key Figure): (i) RNA binding overlaps with the active site and/or cofactor-binding pocket and is in direct competition with substrate or cofactors. If the affinity of the RNA to the ...
The circular dichroism spectra of the mutants were the same as that of the wild-type enzyme. The results from the site-directed mutagenesis demonstrate that the presumed interactions of investigated residues with ATP are important for the stabilization of the transition state. 展开 ...
On the contrary, the binding of the pyridine nucleotide substrate is significantly perturbed demonstrating that the C-terminal potential ADP-binding fold of the beta subunit is indeed the NADPH-binding site of the enzyme. The major effect of the G298A substitution in the GltS beta subunit ...
A drug binding site is a specific location within a protein, such as an enzyme or transporter, where interactions occur between the drug molecule and protein residues, involving hydrogen bonding, van der Waals forces, and hydrophobic interactions, influencing the conformational changes necessary for dr...
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Directed evolution based on saturation mutagenesis at sites lining the binding pocket is a commonly practiced strategy for enhancing or inverting the stereoselectivity of enzymes for use in organic chemistry or biotechnology. However, as the number of residues in a randomization site increases to five ...
It was found that (a) ATP displaces the tRNA from the synthetase with an effector affinity constant corresponding to the Km for ATP of 10 micron; (b) AMP and a number of nucleoside triphosphates, while influencing the rate of aminoacylation, do not displace the tRNA from the enzyme; (c...
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