Dimensions of coordinated and free disulfite are not significantly different; however, the ligand is disordered around a C2 axis passing through the Fe atom and bisecting the S S bond of the chelate ring. This is the first structure of a transition metal disulfite complex. The results suggest ...
The phenomenon of cooperativity was initially observed and studied through the allosteric binding of oxygen to hemoglobin over a century ago (1, 2, 3). A classical model of positive cooperativity is described as a multimeric protein, in which the affinity for ligand binding to an open site on...
The x-ray crystal structures of the cyanide derivative of Lucina pectinata monomeric hemoglobin I (L. pectinata HbI) and sperm whale (Physeter catodon) myoglobin (Mb), generally taken as reference models for monomeric hemoproteins carrying hydrogen sulfide and oxygen, respectively, have been determin...
Transcription factors (TFs) establish patterns of gene expression by binding specific sequences in DNA which are usually 5’ of the target gene and 6 to 12 nt in size [1]. Over 70 families of eukaryotic TFs have been identified and classified based on the structure of their DNA-binding dom...
resistance. Indeed, deletion of the malaria mitochondrial protease PfDegP has been reported to reduce heme levels and DHA susceptibility45. Thus, the detection of PfKelch13 proximal to heme/hemoglobin-containing cellular structures (either cytosolic or membrane-bound) and the evidence of a labile ...
Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes Firefly luciferase is a 62 kDa protein that catalyzes the productionof light. In the presence of MgATP and molecular oxygen, the enzyme oxidizes itssubstra... E Conti,NP Franks,P Brick - 《Structur...
Kinetic data for binding of CO, O2, and tosylmethyl isocyanide to heme cyclophanes when compared to similar data for flat analogues demonstrate that steric effects are manifested primarily in the ligand association rates. This feature of the cyclophane is very similar to observed steric effects in ...
First, the overall quantum yield of the ligand complex is determined primarily by the competition between the rate of iron-ligand bond formation from the initial photoproduct, kB---MbX, and the rate of migration away from state B, kB---C. For example, kB---C approximately equal to 30-1...
DFX mesylate, dissolved in phosphate-buffered saline, was administered by intraperitoneal injection at a dose of 200 mg/kg. Hypoxia was induced in a semipermeable chamber maintained at 5% O2, as determined by a Beckman D2 oxygen analyzer (Beckman Instruments, Irvine, CA), for 6 hours. Mice ...
Its abundant cytoplasmic hemoglobins consist of three single-chain components, each having a moderately high affinity for oxygen (P50 = 0.1–0.2 torr), which is achieved, however, by a very different balance of combination and dissociation rates. Reactions of the monomeric HbI with oxygen are ...