For BiFC studies the cDNAs encoding wild type T14-3c (T14) and the mutant version (T14DN) were cloned into pUCSPYNE and pUC-SPYCE or pSPYNE-35S and pSPYCE-35S, respectively, and transformed into either Arabidopsis protoplasts or tobacco leaf cells. Upon co-expression of T14? Blackwell ...