蛋白质定量检测实验 (Bicinchoninic Acid, BCA) 以Thermo Scientific™ Pierce™ BCA Protein Assay Kits为例,进行蛋白质定量的实验步骤: 1. 材料和试剂: Thermo Scientific™ Pierce™ BCA Protein Assay Kits;待测蛋白质样品;酶标仪;离心机;取样架或微量移液器等 2. 实验步骤: 准备样品: a. 将待测蛋白...
BCA protein assay BCA(Bicinchoninic Acid)蛋白定量试剂盒 BCA蛋白检测 产品介绍: 产品说明 应用 二喹啉甲酸试剂盒被用来估量唾液蛋白组样本,人胚肾293细胞和黄体细胞中的蛋白质。 特点和优势 简单、灵敏的比色法蛋白质检测 两种稳定试剂作为工作溶液 相对于Lowry蛋白检测,速度更快,操作更轻松 ...
Bicinchoninic acid (BCA) assay in low volume[J] . Anthony Bainor,Lyra Chang,Thomas J. McQuade,Brian Webb,Jason E. Gestwicki.Analytical Biochemistry . 2010 (2)Bainor A, Chang L, McQuade TJ, Webb B, Gestwicki JE (2011) Bicinchoninic acid (BCA) assay in low volume. Anal Biochem 410(...
中文名称:蛋白含量测定试剂盒-酶标法(BCA法)(BCA法) 中文同义词:蛋白含量测定试剂盒-酶标法(BCA法)(BCA法) 英文名称:Protein Content Assay Kit(BCA)(Bicinchoninic Acid(BCA)) 英文同义词:Protein Content Assay Kit(BCA)(Bicinchoninic Acid(BCA))
The Bicinchoninic Acid (BCA) Assay For Protein Quantitation John M. Walker 1. Introduction The bicinchoninic acid (BCA) assay, first described by Smith et al. (1) is similar to the Lowry assay, since it also depends on the conversion of Cu2+ to Cu+ under alkaline conditions (see Chapter...
Bicinchoninic acid assay (BCA) method The BCA assay method is based on the fact that the sodium salt of bicinchoninic acid reacts with the cuprous ion generated by the biuret reaction under alkaline conditions. Peptide bonds and some amino acids, such as cysteine, tryptophan, and tyrosine, ...
Bicinchoninic acid; BCA 5g $152.00 RC-128 Bicinchoninic acid; BCA 25g $703.00 Add to Cart Request Quote Description Technical Reviews Citations (8) CoA For complete Bicinchoninic Acid (BCA) protein Assay kits follow the links below: For standard Bicinchoninic Acid (BCA) Protein Assay,click here...
BCA(bicinchoninic acid) BCA蛋白浓度测定试剂盒 全合成法是以人抗体序列为骨架,以鼠抗体的CDR置换人抗体的CDR,将整个可变区序列的两条链分解成若干片段,并使相邻的片段具有彼此互补的粘性末端。合成所有DNA片段,每组片段分别退火,然后逐组连接成完整的可变区基因,插入质粒中,进一步即可用于构建和表达改形抗体。
Bicinchoninic Acid (BCA) Protein Assay (Smith) The bicinchoninic acid (BCA) assay is available in kit form from Pierce (Rockford, Ill.). This procedure is very applicable to microtiter plate methods. The BCA is used for the same reasons the Lowry is used. Stoscheck (1990) has suggested ...
收到该二辛可宁酸 (BCA) 蛋白检测:与非动物蛋白标准品兼容的还原剂后,具体的保存方式查看提供的产品说明书。其他 G-Biosciences是一家专注于蛋白质组学研究的美国公司,产品广泛应用于学术研究和工业制造领域,包括:蛋白质检测、样品制备、电泳、蛋白质印迹、质谱、测定开发、抗体生产和蛋白质纯化等。目前,G-Biosciences...