BHK21-T7/5,BHK21-T7,BHK21T7 传代方法 第一次建议1:2传代 传代情况 2天换液 备注 用无菌离心管收集瓶子培养基,留作过渡对比培养 如果对比培养效果不好,建议直接购买我们的完全培养基 二、细胞收到后处理 培养至良好状态后灌满完全培养液并封好瓶口是运输细胞的最好办法。收到细胞用75%酒精喷洒整个细胞瓶...
形态特征:贴壁生长生长特性:成纤维细胞样特征特性:表达T7-5RNA聚合酶启动子培养条件:RPMI 1640 (w/o Hepes), 10%FBS 1:3～1:4传代,一周1～2次传代情况:C2 冻存条件:基础培养基+8%DMSO+20%FBS 培养法(-) STR:- 同工酶: 染色体: 使用权限:A类图片链接:http://www.cellresource.cn/fcellpicture.aspx...
稳定表达T7RNAP的BHK-21细胞系的建立
BHK-21 cell lines expressing PCI-T7 were developed using conditional medium containing Geneticin (G418) for 2 weeks. A helper DNA plasmid, named as pT7 HN was co-transfected into VT7 cell and detected by Western blot. These results showed that the T7 RNA polymerase of the vaccine virus ...
为建立能稳定表达T7 RNA聚合酶(T7 RNAP)的BHK-21细胞系以研究RNA重组疫苗,本研究从BL21(DE3)大肠杆菌中扩增T7 RNAP基因,定向克隆于质粒pcDNA3.1(+)中,经双酶切及测序鉴定,获得阳性重组质粒pcDNA3.1-T7 RNAP.用该质粒转染BHK-21细胞,经G418筛选14 d后获得阳性细胞株.RT-PCR和western blot检测结果表明,...
We report here on the expression of the hemagglutinin- neuraminidase protein of the Sendai virus using a T7 RNA polymerase system in BHK-21 cell line. The expression was confirmed by the immunoprecipitation and Western/Immunoblott techniques. This HN gene expression has confirmed that the T7 RNA...
RNAs were transcribed from the pACYC- DENV2-Rluc2A replicon in vitro using a mMESSAGE MEGA- script® T7 Kit according to the manufacturer's protocols. BHK- 21 cells transfected with Rlu-DENV-Rep RNAs at MOI=0.1 were seeded into 24-well plates containing 200 μL of culture medium at a...
T7 RNA聚合酶细胞系慢病毒表达系统为了建立能稳定表达T7RNA聚合酶的BHK-21细胞系,利用PCR方法从BL21(DE3)细菌中扩增T7RNA聚合酶基因,并克隆到慢病毒表达系统的pLVX-Puro转移载体上,然后与Lenti-X HTX包装载体共转染Lenti-X 293T细胞系,收获高效价的慢病毒.将慢病毒感染BHK-21细胞,用嘌呤霉素多次筛选后获得抗性...