Tassi C, Mancuso F, Gambelunghe C, Rufini S, Feligioni L, Biondi R, Rossi R, Capodicasa E (2001) Beta- N -acetylhexosaminidase activity and isoenzyme profile in the kidney and urine of trained rats. Immunopharmacol Immunotoxicol 23:573–583...
Supplied lyophilised. Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-β-D-hexosaminides from glycoproteins and oligosaccharides. Specific activity: ~ 2 U/mg (37oC, pH 7.5 on pNP-β-D-N-acetylglucosamine).Stability: Lyophilised powder: > 4 years at -20oC....
This system increases uptake of exogenous enzyme by some neurons in vitro and possibly in vivo, but its efficiency depends on the cells' endocytic activity that, in the case of neuronal soma, apparently is low. Thus, we have chosen as recognition marker for specific neuronal uptake a nontoxic...
With the thioethyl β-lactoside as acceptor, ja:math was formed, owing to the action of residual ja:math activity in the N -acetylhexosaminidase on the thioethyl β-lactoside acting as both donor and acceptor. 展开 关键词: N-acetylhexosaminidase trisaccharides N-acetylgalactosaminide transfer ...
TorticollisWriter's crampSpecific activities of β- d -hexosaminidase, α- d -mannosidase, β- d -galactosidase and β- d -glucuronidase were determined in fibroblasts of patients with writer's cramp and torticollis. These diseases show degenerative neurological disorders similar to those observed in...
NEW MATERIAL:beta-N-acetylhexosaminidase having a molecular weight of 56,000+ or -2,000 and having the following physical and chemical properties. Activity, active to natural substrate (e.g. chitin and N-acetylchitooligosaccharide) and synthetic substrate (e.g. p-nitrophenyl N-acetyl-beta-D-...
PROBLEM TO BE SOLVED: To provide a DNA encoding a protein having β-N-acetylhexosaminidase activity.NOMURA TAKESHI野村 雄ISHIYAMA SEIJI石山 誠司IKEDA SHINKO池田 真弘USAMI AKIHIRO宇佐美 昭宏FUJIYAMA KAZUHITO藤山 和仁beta-N-acetylhexosaminidase. EC 3.2.1.52.http://www.chem.qmul.ac.uk/iubmb/...
The enzyme showed about 1.5-fold higher β- N -acetylgalactosaminidase activity than β- N -acetylglucosaminidase activity. The two activities could not be separated by any process and their ratio remained almost constant throughout the whole purification procedure. The molecular weight was ...
The enzyme activity was inhibited by SH-reagents, indicating that it contains a SH-group in its active site. The enzyme has a high affinity for pNPGlcNAc with Km value of 0.021 mM. The rate of hydrolysis of N-acetylchito-oligosaccharides tended to decrease with increasing degree of ...
Optimal pH and temperature for beta-N-Acetylhexosaminidase activity were 5.0 and 60 degrees C, respectively. Enzyme activity was inhibited by sodium selenate (Na(2)SeO(4)), mercuric chloride (HgCl(2)) and sodium dodecyl sulfate (C(12)H(25)SO(4)Na), and activated by zinc, calcium, ...