Majority of ?-glucosidase producers during non-monsoon belonged to the members of Bacillales (53%), Pseudomonadales (26%), and Vibrionales (11%) which shifted to the members of Enterobacteriales (51%), Bacillales (14%), Alteromonadales (12%), Aeromonadales (9%), Xanthomonadales (...
Phytochemical Analysis 12 : 174–179.beta-Glucosidase and peroxidase stability in crude enzyme extracts from green beans of Vanilla planifolia Andrews. Dignum M J,Kerler J,Verpoorte R. Phytochemical analysis : PCA . 2001Dignum M J, Kerler J, Verpoorte R. Beta -glucosidase and peroxidase stability...
Use of model organisms and in vitro techniques relevant to developmental biology and medicine are acceptable, as are translational human studies.doi:10.1203/00006450-197804001-00574Wenqer, David AOlson, GrantPediatric Research
In liver and spleen, enzyme activity peaked at about 20 min postinjection followed by a biphasic decrease with t1/2 approximately 40-60 min and approximately 12-14 h. In bone marrow maximal enzyme activity was at 40-60 min with a disappearance t1/2 approximately 60 min. Quantitative cross-...
12. The method of claim 11, wherein the pretreating comprises contacting the biomass composition with dilute acid. 13. A method of producing ethanol, said method comprising: a) contacting a biomass composition with the enzymatic composition of claim 1 and a fermentative microorganism; and b) cul...
12 and SEQ ID NO: 14; - an amino acid sequence that has an identity of at least 90%, preferably 95%, 98% or 99%, with the sequence SEQ ID NO: 2 and that comprises the modification Q225H; - an amino acid sequence which has an identity of at least 90%, preferably 95%, 98% ...
P. Chen, X. Fu, T. B. Ng, and X.-Y. Ye, "Expression of a secretory 𝛽-glucosidase from Trichoderma reesei in Pichia pastoris and its characterization," Biotechnology Letters, vol. 33, no. 12, pp. 2475- 2479, 2011.Chen P, Fu X, Ng TB, Ye XY. Expression of a ...
It had optimum effect at pH 3.5-4.0, whilst optimum temperature was 20 deg C, compatible with typical wine-cellar conditions; it was not inhibited by ethanol, at concentrations of 12-14%, or fructose and glucose. The betaG was also characterised in terms of the kinetic parameters K_m (...
The PcbglB was also intracellularly expressed in combination with the lac12 lactose permease of Kluyveromyces lactis in S. cerevisiae Y294[PcbglB + Lac12]. The recombinant extracellular beta-glucosidases indicated maximum activity in the pH range 4-5 and temperature optima varying from 50 to 75 ...
is smaller than a corresponding reference amino acid residue in a corresponding GH3 BGL reference coordinating loop domain comprising a sequence as set forth in GLDMX1refMPX2refX3refX4refX5refX6refX7refX8refX9refX19refX11refX12refX13refX14refX15refX16ref (SEQ ID NO: 252); (c) a GH3 BGL 尾...