CONSTITUTION:A Thermus bacterium such as Thermus thermophilus HB8 is aerobically cultured in a medium containing yeast extract, pepton, glucose and other. The cells are collected, crushed, extracted to give beta-galactosidase. The resultant beta-galactosidase has following physical and chemical ...
β-Galactosidases with substitutions for His-540 were only poorly reactive with galactosyl substrates. However, the activity with substrates that were like galactose but did not have a C6 hydroxyl group was not decreased much as a result of such substitutions. The loss of transition state stabiliza...
beta-Galactosidase (EC 3.2.1.23) from fungus Curvularia inaequalis was modified by active brilliant orange KH and adsorbed on DEAE-Sephadex A-50. The lactose hydrolysis was studied in a continous flow on the column packed with the immobilized enzyme. The pH and temperatures optima for the substr...
上海玉博生物科技有限公司 主要致力于“Rabbit polyclonal to beta Galactosidase Abcam ab4761 ”的生产销售。多年的“Rabbit polyclonal to beta Galactosidase Abcam ab4761 ”生产与销售的经验,与各行业新老用户建立了稳定的合作关系,我公司经营的产品名称深受广大
- 1990 - 364-365 - www.sciencedirect.comdoi:10.1016/0734-9750(90)90840-8Gibbons, KathrynHalvorsen, ChristianSiring, ErlingELSEVIERBiotechnology Advances
In transgenic mice, Eyer and Petersen =-=(8)-=- expressed an NFH/�-galactosidase fusion protein in which the COOH terminus of NF-H was replaced by �-galactosidase. NF inclusions were found in the perikarya of neurons and the resulting NF aggregate......
被引量: 8发表: 2011年 Genetic Engineering of a Radiation-Resistant Bacterium for - OSTI including vectors for general cloning,expression, and promoter analysis with the same two reporters, catechol diooxygenase (XylE) andbeta-galactosidase (LacZ... AA Subset 被引量: 0发表: 2014年 ...
Beta-Galactosidase [EC 3.2.1.23] has been purified from a culture of Aspergillus oryzae by 2-propanol fractionation, column chromatography on DEAE-Sephadex A-50 and Sephadex G-200. The preparation was homogeneous on ultracentrifugation and disc electrophoresis. The enzyme showed pH optima of 4.5 wit...
et al., " Isolation and Characterization of a Keratan Sulfate-Degrading Endo-beta-Galactosidase from Flavobacterium Keratolyticus ", 25 April 1981, Vol. 256, No. 8, pages 3906-3909.Kitamikado, M., Ito, M. & Li, Y.-T. (1981) Isolation and charac- terization of a keratan sulfate-...
The enzyme β-galactosidase (EC 3.2.1.23) from Aspergillus niger was purified and resolved into three multiple forms, using molecular sieving, ion-exchange, and hydrophobic chromatography. The isolated enzyme forms accounted for 8...