(2~7)It has become apparent that anticardiolipin antibody from patients with anti- phospholipid syndrome (APS) recognize a modified β2GP1 structure and not cardiolipin, native β2GP1 or an epitope structurally defined by both cardiolipinCatalog No.E...
[3]. This assay involves coating purified human β2GPI on the surface of an irradiated plate. The patient serum is then added and the presence of IgG or IgM anti-β2GPI antibodies detected by the addition of a human anti-IgG (or anti-IgM) antibody conjugated with an enzyme that react...
Twenty-seven patients (60%) had clinical manifestations of antiphospholipid antibody syndrome. Nineteen patients (42.2%) had a history of thrombosis, and eight (17.7%) had a history of recurrent fetal loss. aBeta(2)-GP1 was (IgG) was positive in 23 (51.1%), aCL in 13 (28.8%), and ...
Affinity purified murine IgG2 anti-β2 GPI monoclonal antibody (moAb) (4B2E7) and affinity purified rabbit polyclonal anti-β2 GPI antibody were produced as previously described30,31. Isotype control murine IgG2 and rab- bit polyclonal IgG and 70 μm nylon mesh were purchased ...
The effects of (a) purified anti-beta-2 glycoprotein 1 antibodies, (b) anti-beta-2 glycoprotein 1 antibody-depleted patient IgG, and (c) IgG from normal human sera on thrombus formation were studied quantitatively in the live mouse. Intravital microscopy was performed using the cremaster muscle...
Cardiolipin+beta2GPI induced the highest levels of anti-beta2GPI and anti-CL IgG Ab and lupus anticoagulant (LA) activity, while beta2GPI with PC or PS/PC vesicles produced no significant anti-PL Ab. PS+beta2GPI was somewhat immunogenic, but less so than PG+beta2GPI. beta2GPI was ...
Isolation of Mgat1 knockout clones in an IgG -producing CHO cell line ZFN-mediated knockout of Mgat1 was employed to create cells with a homogenous, simple N-glycoform profile containing terminal Man5 moieties. ZFNs targeting CHO exon 4, the only coding exon, were designed (Fig. 1) and ...
AntibodyAutoimmunityTherapyThe complement system is a key part of the innate immune system and plays an important role in the clearance of pathogens and apoptotic cells upon its activation. It is well known that both IgG and IgM can activate complement via the classical pathway by binding of C1q...
Conclusion : the anti-beta-2 glycoprotein 1 assay, requiring only 5μl serum, may be a useful addition to antiphospholipid-antibody diagnostics in cases of paediatric stroke.doi:10.1007/s00431-003-1285-9Freja EbelingJari PetjSoile Alanko
Both patients were treated with therapeutic plasma exchange (TPE), which resulted in a marked decrease in antibody titer accompanied by an improved clinical outcome in one patient (IgG antibody). In the second patient, the outcome was poor despite TPE (IgA antibody). There were no significant ...