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Individual immunoblots were probed with antibodies to NOTCH1, NCSTN, PEN2, PSEN1 and PSEN2 (Cell Signalling, Danvers, MA) diluted 1:1000; to PDX1 (Upstate) diluted 1:3000; to MAFA (Santa cruz,) diluted 1:800; to NEUROD1 (abcam) diluted 1:3000. β- tubulin antibody (Sigma-...
Interestingly, recent reports suggested that anticancer mechanism of BS is similar to the activity of colchicines in binding preferentially on β-tubulin isotypes such as βII and βIII in the αβ-tubulin dimer37. Both the SEM and phase contrast analysis revealed that large number of mitotic c...
上海玉博生物科技有限公司 主要致力于“PIERCEMA119187Anti-beta-3 Tubulin Monoclonal Antibody100 µg 100 µg”的生产销售。多年的“PIERCEMA119187Anti-beta-3 Tubulin Monoclonal Antibody100 µg 100 µg”生产与销售的经验,与各行业新老用户建立了稳定的合
(a) Western blot analysis of whole cell lysates from MCF-7, T-47D and SK-BR-3 cells was carried out using antibodies to GABPα, GABPβ and the blots were then reprobed with anti-γ-tubulin as an internal control. Apparent molecular weight markers (kDa) are indicated to the right of...
Ectoderm: β-III-tubulinEndoderm: α-fetoprotein Fig. 1 panel GI Donor screening (OPTIONAL) HIV 1 + 2 Hepatitis B, Hepatitis C N/A N/A Genotype additional info (OPTIONAL) Blood group genotyping N/A N/A HLA tissue typing N/A N/A A guide RNA was designed to target PCCB exon 2 (...
(a) Western blot analysis of whole cell lysates from MCF-7, T-47D and SK-BR-3 cells was carried out using antibodies to GABPα, GABPβ and the blots were then reprobed with anti-γ-tubulin as an internal control. Apparent molecular weight markers (kDa) are indicated to the right of...
both blots were stripped and confirmed for absence of signal. Blots were then re-probed with an anti-LRP-1 antibody which recognizes the small subunit (Epitomics Burlingame, CA, USA; 0.2 μg/ml), washed, and probed with anti-rabbit secondary (Santa Cruz Biotechnology, Inc., Santa Cruz...
(BD 610843; IB 1:1000), smooth muscleα-actin (Sigma A2547; IB 1:2000, IHC 1:400), smooth muscle myosin heavy chain 11 (abcam ab53219; IB 1:1000 for mouse cells), smoothelin (Millipore MAB3242; IB 1:1000), TGFβR1 (Santa Cruz sc-398; IB 1:1000), andβ-tubulin (Sigma T...
*Po0.05 versus control; **Po0.05 versus L-BMAA alone and rSOD1G93A monoclonal β-tubulin antibody was from Sigma-Aldrich. Western blotting, ECL reagents and chemicals were from GE Healthcare (Milan, Italy). The inactive mutant HA-Aktk179M (Akt D − ) plasmids were donated by P. ...