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Competing interests MK and BE declare stock options in O2M Technologies, LLC. All other authors have no competing interests. Additional information Correspondence and requests for materials should be addressed to M.K. Reprints and permissions information is available at www.nature.com/reprints. ...
Oil Red O working solution was prepared by mixing 6 parts of Oil Red O stock solution with 4 parts of water (by volume), which was cooled to room temperature and filtered through 0.2 μm filters. For staining, cells were washed with PBS, fixed panol for 5 min. After 5 min...
Beta titanium alloys were recognized as a distinct materials class in the 1950s, and following the introduction of Ti-13V-11Cr-3Al in the early 1960s, inte
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sTRAIL cDNA was synthesized using custom gene synthesis from Integrated DNA Technologies (IDT; Coralville, IA). The empty pN3-Control backbone was a gift from Guntram Suske (Addgene plasmid # 24544). sTRAIL cDNAwas cloned into pN3-control backbone by restriction enzyme digest and amplified ...
Proteins with a metallo-beta-lactamase (MBL) fold have been largely studied in bacteria in the framework of resistance to beta-lactams, but their spectrum of activities is broader. We show here that the giant Tupanvirus also encodes a MBL fold-protein that has orthologs in other giant viruses...
Site-directed mutagenesis was performed using the Quickchange Site-Directed Mutagenesis Kit (Agilent Technologies) according to the manufacturer's instructions. pET28-P99 was used as a template, and the synthetic oligonucleotide primers were P99_A266C+(SEQ ID No. 3) and P99_A266C− (SEQ ID...
Aβ1–40 was prepared as a 0.5-mM (1 mg/460 μL) stock solution in Milli-Q water and filtered through a 0.22-μm filter (Millipore, Billerica, MA, USA). The solution was held at 4°C for 60 hours and then incubated at 37°C for 8 hours with gentle mixing every 2 hours to ...
(24-ethyl-5-cholestene-3-ol, CAS number 83-46-5, 95%) was purchased from Heowns Biochem Technologies, Llc. (Tianjin, China). The principles of laboratory animal care were followed throughout the duration of experiment and instructions were provided by the Animal Experimental Ethics Committee ...