The present invention relates to the use of a nucleic acid fragment or part thereof encoding β-ketoacyl ACP synthase II (KASII), particularly which derived from to enhance the production and the accumulation of very long chain fatty acids (VLCFA) in a plant, oil crops of particular.UMI ...
beta-Ketoacyl-[acyl carrier protein (ACP)] synthase forms new carbon-carbon bonds in three steps: transfer of an acyl primer from ACP to the enzyme, decarboxylation of the elongating substrate and its condensation with the acyl primer substrate. Six residues of Escherichia coli beta-ketoacyl-ACP...
Beta-Ketoacyl-acyl carrier protein (ACP) synthetase catalyzes the condensation reaction of fatty acid synthesis in Escherichia coli. The homogeneous enzyme reacts with hexanoyl-CoA to form hexanoyl-enzyme which was isolated and characterized. Hexanoyl-enzyme contains 2 mol of hexanoate/mol of enzyme ...
By this invention, compositions and methods of use related to 尾-ketoacyl-ACP synthases. Of special interest are synthases obtainable from Cuphea species. Amino acid and nucleic acid for synthase protein factors are provided, as well as methods to utilize such sequences in constructs for ...
β-Ketoacyl acyl carrier protein (ACP) synthetase, which catalyzes the condensation of fatty acyl-ACP with malonyl-ACP to form β-ketoacyl-ACP, has been crystallized in a highly active form which is stable for several months. Phosphate has been shown to cause enzyme activation which may be ...
Acyl carrier protein (ACP) is a required cofactor for fatty acid synthesis in Escherichia coli. Mutants lacking beta-ketoacyl-ACP synthase II activity (fabF1 or fabF3) possessed a different molecular species of ACP (F-ACP) that was separated from the normal form of the protein by conformation...
β-Ketoacyl-[acyl-carrier-protein] (ACP) reductase, β-hydroxyacyl-ACP dehydrase, and enoyl-ACP reductase have been purified to homogeneity from extracts of spinach leaves. Based on sodium dodecyl sulfate-polyacrylamide gel eletrophoresis studies, the monomeric molecular weights of the β-ketoacyl-...
Crystal structure of β-ketoacyl-acyl carrier protein synthase II from E.coli reveals the molecular architecture of condensing enzymes In the biosynthesis of fatty acids, the β-ketoacyl-acyl carrier protein (ACP) synthases catalyze chain elongation by the addition of two-carbon units derived from...
Here we describe the crystal structures of the Yersinia pestis β -ketoacyl-ACP synthases FabF and FabH, and compare these with the unpublished, deposited structure of FabB, presenting three promising targets for the development of new antimicrobials to combat MDR Y. pestis strains. Materials ...
Beta-Ketoacyl Synthase-Chain Length Factor beta-ketoacyl-ACP reductase Beta-ketoacyl-ACP synthase Beta-Ketoacyl-ACP Synthase I beta-ketobutyric acid beta-ketobutyric acid beta-ketobutyric acid beta-ketobutyric acid beta-ketobutyric acid beta-ketohydrogenase ...