yujiaping1 你的实验目的是什么,为什么需要基因组序列,cDNA序列已经是基因的全序列了,对于一般的实验设计引物应该足够了,如果你需要跨内含子设计引物,可能是需要基因组序列, snoopyzxx 看家基因啊,引物网上搜搜就出来了吧,还要自己设计? 彬心玉壶 不用自己设计,多看几篇文献,上面自然会有,太常见的内参引物了。猜...
PCR Method2-step RT-qPCR Gene SymbolACTB Green FeaturesSustainable packaging Label or DyeVIC Product LineTaqMan™, VIC™ Quantity2500 reactions Shipping ConditionRoom Temperature SpeciesHuman Targetβ-actin For Use With (Application)Gene Expression Concentration20X No. of Reactions2500 Reactions Unit ...
PCR Method2-step RT-qPCR Gene SymbolACTB Green FeaturesSustainable packaging Label or DyeFAM Product LineFAM™, TaqMan™ Quantity2500 reactions Shipping ConditionRoom Temperature SpeciesMouse Targetβ-actin For Use With (Application)Gene Expression Concentration20X No. of Reactions2500 Reactions Unit ...
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a maj...
实时定量PCR(Real-time reverse transcription-quantitative PCR,RT-qPCR)流程 8.1 目的基因引物的合成 引物由北京赛百盛公司合成,引物序列或货号如下: Gene Primer Sequences Wnt3a 上游 下游β-catenin 上游下游 BDNF 上游 下游 PCR标本的留取 5’-TGCTGTTGAGGCAATGGTC-3’ 5’-TGGCAGATGGGCTGTATGTA -3’ 5...
(6)mRNA表达(20μl反应体系):ABI7500PCR仪上进行mRNA表达:96孔qPCR反应板,3μlcDNA,10μlSYBRGreenⅠ,5μlddH2O,2μlPrimers(所用引物序列见下表1)。PCR扩增后进行溶解曲线观察分析非特异性扩增和引物二聚体是否存在,Ct值用2∧-△△Ct法计算基因表达量差异,以18S为内参照基因。表1引物序列详细信息引物...
2.6实时荧光定量pcr检测各样品的内质网应激相关(bip、xbp1s、chop、atf4)rna水平:反应体系(10μl):cdna模板1μl,qpcrmastermix(promega)5μl,正反引物0.4μl,depc处理的三蒸水3.2μl。混匀后,95℃10分钟预变性,95℃15秒,60℃1分钟(40个循环)。
beta Actin rabbit polyclonal antibody Protein full name Actin, cytoplasmic 1 Synonyms ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta Cytoskeletal Actin Immunogen KLH conjugated Synthetic peptide corresponding to Mouse β-Actin Isotype