The cleaved-off different short molecules may undergo complete hydrolysis: ① acetyl xylan esterase removes the terminal acetate residues by break the ester linkage, α-D-glucuronidase attacks the α-1,2-glycosidic bond and releases the glucuronic acid side chain, and β-xylosidase hydrolyzes the ...
β-Glucosidases are a class of enzymes (EC: 3.2.1.21) that can hydrolyze the terminal, nonreducing β-D-glucosyl residues by hydrolyzing the β-1,4 glycosidic bond of various glycoconjugates including glucosides, oligosaccharides, and 1-O-glucosyl esters, to form glucose. ...
Mechanistic insights into glycosidic bond activation in cellulose pyrolysis were obtained via first-principles density functional theory calculations that explain the peculiar similarity in kinetics for different stereochemical glycosidic bonds (beta vs alpha) and establish the role of the three-dimensional ...
Cellulose is composed of β-D-glucan units linked by (1→4)glycosidic bondsthat are formed by a simple polymerization of glucose residues from a substrate such as UDP-glucose (O'Sullivan, 1997;Delmer and Amor, 1995). The stereochemistry conferred by the glycosidic linkage creates a linear exte...
In particular, multiple α- and β-glucans like cellulose, dextran, curdlan and cyclic α- and β-glucan, among others, have been produced in bacteria [2]. Compared to other polysaccharides, CβG have the advantage of not requiring a primer or acceptor substrate for its synthesis since Cgs...
Kluyveromyces fragilisCovalent coupling activated by epichlorohydrinCellulose beadsFluidised-bed reactor>90% conversion in 5h[84] β-GalactosidaseCore-shell microcapsulationAlginate–chitosan alginate core cross-linked with Ca2+and Ba2+ionsND–[85] ...
(2007; 2009) purified two XET isoenzymes from extracts of barley seedlings to a near monodisperse form and showed that the barley HvXET5 and HvXET6 enzymes catalyse the in vitro formation of covalent linkages between xyloglucan oligosaccharide acceptors and celluloses, and between xyloglucan ...
Enzymes involved in cellulose hydrolysis are often inhibited by their end-products, cellobiose and glucose. Efforts have been made to produce more efficient enzyme variants that are highly tolerant to product accumulation; however, further improvements are still necessary. Based on an alternative ...
. Chitin, the second most abundant natural polymer, after cellulose, is a linear polysaccharide of GlcNAc linked by β-1,4-glycosidic bonds (Lv et al. Highly efficient and selective biocatalytic production of glucosamine from chitin. Green Chem., 2017, 19, 527-535). The degradation products ...
The term “cellulase” refers to a category of enzymes capable of hydrolyzing cellulose (β-1,4-glucan or β-D-glucosidic linkages) to shorter oligosaccharides, cellobiose and/or glucose. Cellulases include 1,4-β-D-glucan glucanohydrolase (“endoglucanase” or “EG”); 1,4-β-D-glucan ce...