5. After the amplification is complete, use 5 - 10 μl of the reaction for agarose gel electrophoresis to verify the amplified DNA fragments. The PCR amplified product can frozen until subsequent analysis.【 Amplified DNA size when using the supplied Positive Control RNA 】...
DNA extraction, library preparation and sequencing We extracted DNA from fin clips using a salting-out protocol modified from Sunnucks and Hales (1996) (S1b). DNA was assessed for quality using a NanoDrop spectrophotometer (Thermo Scientific), for integrity using gel electrophoresis (agarose, 2%)...
RNA concentration and quality were verified using the Qubit RNA assay (Life Technologies) and electrophoresis using a 1.2% agarose gel under denaturing conditions. First strand cDNA for each sample was synthesized from 1 μ g total RNA using the iScript supermix (Biorad Laboratories). qPCR ...
To remove adapter dimers, many kits recommend a gel purification approach, in which pooled sequencing libraries are run on an agarose gel containing a DNA intercalating dye (e.g. ethidium bromide). The ~300 bp library band is carefully – but quickly – excised under UV light using a small...
(Life Technologies). RNA concentration and quality were verified using the Qubit RNA assay (Life Technologies) and electrophoresis using a 1.2% agarose gel under denaturing conditions. First strand cDNA for each sample was synthesized from 1 μg total RNA using the iScript supermix (Biorad ...
QIAGEN胶回收试剂盒QIAquick Gel Extraction Kit(28704) 货号:28704 QIAGEN琼脂糖Ni-NTA Agarose(30230) 货号:30230 QIAGEN裂解缓冲液Buffer RLT(79216) 货号:79216 QIAGEN转染试剂Effectene Transfection Reagent(301425) 货号:301425 QIAGEN裂解液Buffer TCL, 2x(1070498) ...