Marination, the focus of the present study, is a process whereby meat is treated with a liquid solution for just a few minutes or for up to several days. The liquid solution, which typically consists of a blend of acids, salts, oils, and spices, can be applied to the meat via immersio...
On d 0 (30 days after weaning), heifers were administered two 5-mL i.m. injections of porcine red blood cells (PRBC; Lampire Biological Laboratories, Pipersville, PA) solution (25% PRBC and 75% sterile PBS; Amresco, Solon, OH), concurrent to either a single 2.5-mL s.c. injection ...
2.4. DNA Extraction, PCR Amplification, and Sequencing Total DNA was extracted from the rumen fluid samples of 45 Angus steers (15 for each treatment) by using the FastDNA® SPIN for Soil kit (MP Biomedicals, Solon, OH, USA), according to the manufacturer's protocol. The quality of all...