For T-cell response evaluation, a skin biopsy acquired sufficient skin-infiltrating T-cells (SKILs) in 10 out of 13 tested patients, one week after the fourth dose of the vaccine. Among these patients, treatment-specific CD8 + T-cells were observed in 4 individuals. Among patients withou...
In order to design these vaccines, firstly, tumor mutations should be detected. After tumor biopsy and whole-exome sequencing, non-synonymous mutations are detected through comparison with whole-exome sequencing of non-cancerous cells of the affected individual. Then TSAs are ranked based on their...
However, the types of mutations detected and the resulting mutational signatures in colon and small intestine cells were different from those in liver cells. To be pointed out, the inter-individual variation in mutation rate and spectra are low, indicating organ-specific activity of common ...