五、LHC-9(中科院) (6) 1.Transforming growth factor-β impairs glucocorticoid activity in the A549 lung adenocarcinoma cell line BEAS-2B cells (from ATCC, Manassas, VA, USA) were cultured in LHC-9 media containing 2% vv?1 heat-inactivated FCS, 15 mM HEPES, 0.2% vv?1 sodium bicarbonate,...
BEAS-2B cells (from ATCC, Manassas, VA, USA) were cultured inLHC-9 mediacontaining 2% vv−1heat-inactivated FCS, 15 mM HEPES, 0.2% vv−1sodium bicarbonate, 2 mM L-glutamine, 50 IU·mL−1penicillin and 50µg·mL−1streptomycin and maintained at 37°C in a humidified 5% CO2/...
(GIBCO, Grand Island, NY) (“complete culture media”). The cells were maintained in 25 cm2 polystyrene tissue culture flasks in an incubator at 37°C with 5% CO2 and 100% humidity. The media were changed every 2 days, and the
吲哚培养基培养基,用于细菌靛基质试验,亦称色氨酸肉汤(SN标准)incubationmedia吲哚培养基培养基,用于细菌靛基质试验,亦称色氨酸肉汤(SN标准) MKTTnBroth 临床检验培养基 Brain-heart agar脑心琼脂1.13825.0500 MERCK默克incubation media Brain-heart agar脑心琼脂1.13825.0500 MERCK默克 ...
大家是不是感觉液体培养基会贵很多,以前是这样,但现在大家都认同了;无血清培养基(Serum-Free Media),通常以SFM表示,顾名思义,就是在细胞培养中不需要添加血清,但是在某些应用中可能要添加生长因子或细胞因子。无血清培养基中添加了血清的主要成分:粘附因子、生长因子、必需的营养物质和激素等,能减少上述血清带来的...
(a)P] to the culture media to get the negative and positive controls respectively;the BEAS-2B cells treated with CTPE exposure or RNAi technique were the observational groups.The malignancy of cells was estimated by soft agar colony formation assay.The genetic and protein expressions ...
The relative responses of BEAS-2B cells in the two types of cell culture media are characteristic of what we observed in a separate study comparing differentin vitrolung cell models for particle toxicology [manuscript in preparation]. BEAS-2B cells in KGM media are highly responsive to vanadium ...
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European Journal of Surgical Oncology, Volume 48, Issue 7, 2022, pp. 1527-1535 Anne-Rose W. Schut,…, Erik A.C. Wiemer View PDFShow 3 more articles Article Metrics Citations Citation Indexes5 Captures Mendeley Readers17 Social Media Shares, Likes & Comments1 View details ...
使用软件 Gel-Pro Analyzer(Gelpro32,美国 Media Cybernetics公司)对条带进行灰度分析。 1.2.6 数据统计分析 文中所述实验结果至少重复3次。对照组原始数据做归一化处理,数据显示为平均值±标准偏差。使用SPSS软件(SPSS 13.0,美国SPSS公司)对组间差异进行统计分析,使用单因素方差分析法(One-way analysis of variance...