The findings suggest that bead-beating DNA extraction can enhance the accuracy of PCR-based genetic diagnosis for PJI caused by Gram-positive bacteria.Hieda, YutaChoe, HyonminIke, HiroyukiAbe, KokiKumagai, KenTakeyama, MasanobuKawabata, Yusuke...
Evaluation of real-time qPCR-based methods to detect the DNA of the three protozoan parasites Cryptosporidium parvum, Giardia duodenalis and Toxoplasma gon... But trypsin digestion and direct DNA extraction by bead-beating and FastPrep homogenizer achieved higher performance (e.g. eLD95METH: 4-400...
fecal samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. Zymo MagBinding Bead technology, which features Zymo Research's Inhibitor Removal technology, is then used to isolate the DNA. Eluted DNA is ideal for downstream molecular-...
Firstly, bacteria lysis was innovatively achieved by rotating a pair of magnets to generate bead-beating while the chip was kept stationary during lysis, which simplified the chip design because no additional valve was needed. Secondly, the on-chip assay time was short (within 70 min), which...
Increase protein, DNA and RNA yields with the Bead Ruptor 24 Elite The Bead Ruptor 24 Elite unique tube carriage motion coupled with sample specific bead beating materials 24 Elite results in a increase in nucleic acid, protein and small molecules yields and increases the sensitivity of the downs...
Lactobacillius acidophilus(L. acid.) obtained from MicroKwik vials (Carolina Biological Supply, Burlington, NC, USA) was cultured in de Man, Rogosa and Sharpe (MRS) agar formulated in-house using Millipore-Sigma formulation (CCW4691). The QuickExtractTM one-step DNA extraction kit (Lucigen, ...
Prion Extraction Methods: Comparison of Bead Beating, Ultrasonic Disruption, and Repeated Freeze-Thaw Methodologies for the Recovery of Functional Renilla-Prion Fusion Protein from Bacteriaprimary preventioncore stabilizationpatient educationincidencelow back painMolecular DNA technology allows for production of ...
Andersen LO, Roser D, Nejsum P, Nielsen HV, Stensvold CR (2013) Is supplementary bead beating for DNA extraction from nematode eggs by use of the NucliSENS easyMag protocol nec- essary? J Clin Microbiol 51(4):1345-1347Andersen LO, Roser D, Nejsum P, Nielsen HV, Stensvold CR: Is ...
Bead beatingAerationFine-pore diffusersFragment length amplificationWastewaterAn existing bead-beating DNA extraction protocol was employed to compare the DNA extraction recovery and fragment quality of 6 different aeration diffuser biofilms. Escherichia coli, Gordonia amarae, and mixed liquor were used as ...
Optimizing an enclosed bead beating extraction method for microbial and fish environmental DNAbeadsbony fisheseDNA extractionmarine lifemicrobesSterivexdoi:10.1002/edn3.251Sean R. AndersonLuke R. ThompsonEnvironmental DNA