BD Phosflow" Lyse/Fix Buffer is intended to be used for the lysing and fixing of whole blood for use with intracellularflow cytometry. 产品介绍 下单购买 下单前请务必联系店铺客服,提供全程优质服务。 查询产品访问优宁维商城:https://www.univ-bio.com/,...
2. 用去离子水或蒸馏水(室温)稀释所需量的BD Phosflow™Lyse/Fix Buffer (5X浓缩液)1:5,然后将溶液预热至37°C。每次实验时,应将1X工作溶液制成新鲜溶液,实验结束时应丢弃任何剩余溶液。 3.将一卷血液与20卷预热的BD Phosflow™Lyse/Fix Buffer (1X)混合。通过大力翻转试管8-10次混合均匀,然后将试管...
不能渗透核膜。所以我们建议使用BDPhosflowpermbuffers。在不需要裂解红细胞的情况下,BDCytofix fixationbuffer(Cat.No.554655)可用于protocolsII或III的固定。如果需要裂解红细胞,我们推荐使用 BDPhosflowLyse/FixBuffer(Cat.No.558049)进行细胞的裂解固定。
566349 BD Horizon™ Brilliant Stain Buffer 1000 Tests 566385 BD Horizon™ Brilliant Stain Buffer Plus 1000 Tests 558049 BD Phosflow™ Lyse/Fix Buffer 5X 250 mL 553057 BD Pharmingen™ Purified NA/LE Hamster 145-2C11Anti-Mouse CD3e 0.5 mg 553294 BD Pharmingen™ Purified NA/LE Ham...
558050 BD Phosflow™ Perm Buffer III 125 mL 554722 BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution 125 mL 554655 BD Cytofix™ Fixation Buffer 100 mL 554724 BD GolgiStop™ Protein Transport Inhibitor (Containing Monensin) 0.7 mL 555029 BD GolgiPlug™ Protein Transport Inhibito...
BD Phosflow Lyse/Fix Buffer 5X推荐供应商 我要询价 碧迪医疗器械(上海)有限公司 联系电话:01-400819 65666427 电子邮件:BDB.SEA.Marketing@bd.com CB指数:58 网址:www.bdbiosciences.com/ 相关信息:产品目录(13772) 用户评价 英文名称:BD Phosflow Lyse/Fix Buffer 5X ...
This antibody conjugate is suitable for intracellular staining of human lymphoid cell lines, peripheral blood mononuclear cells, and mouse splenocytes using BD Cytofix™ Fixation Buffer or BD Phosflow™ Lyse/Fix Buffer with BD Phosflow™ Perm Buffer III (see table, below). Prior to stimulati...
BD Pharmingen stain buffer染色缓冲液(FBS)可用于淋巴组织、外周血或培养细胞制备的单细胞悬液的免疫荧光染色。染色缓冲液(FBS)对荧光试剂的稀释和应用以及用于流式细胞仪分析的细胞的悬浮液、洗涤和存储都是有用的。基于之前对染色培养基的报道,染色缓冲液(FBS)被配制成中性pH (pH 7.4)缓冲盐溶液(即DPBS),其中...
557885 PHOSFLOW PERM/WASH BUFFER I 125ML 557891 BRDU FITC FLOW KIT 4X50TST 557892 BRDU APC FLOW KIT 4X50TST 557895 HU CD15 PURE MAB 0.1MG W6D3 557896 MS CD14 NALE MAB 0.5MG 4C1/CD14 557899 R-PE IMAG PRTCL DM 5.0ML E31-1459 557903 MS IGG2B KPA ITCL ALEXA 647 100TST 27-35 557...
b.Obtain whole blood or suspend spleen cells. Treat the cells (eg, 100 µL) with the desired activator and/or inhibitor. After treatment, add a 20-fold excess volume (eg, 2.0 ml) of warmed BD Phosflow™ Lyse/Fix Buffer. Mix well and incubate the tubes in a 37°C water bath for...