Human BCL2L2 qPCR Primer Pair 一键复制产品信息 产品编号:QH01417S 产品包装:200次 选择包装 200次 数 量 价格:¥21.00
Mouse Bcl2l2 qPCR Primer Pair 一键复制产品信息 产品编号:QM01330S 产品包装:200次 选择包装 200次 数 量 价格:¥21.00
Several selected genes from Hippo pathway were validated by RT-qPCR in independent experiments of transient Bcl-2 silencing in A375 cells, confirming the downregulation of TEAD4, TP73, CCND3, EAD2, and MYC (Fig. 2A). Fig. 2 Validation of genes modulated by Bcl-2, enriched by KEGG and...
To detect mRNA, qPCR was performed as described before [47] using following primers: Mcl-1: MCL1for (TCTCATTTCTTTGGGTGCCTTT), MCL1rev (GATATGCCAAACCAGCTCCTAC); House keeping gene β2-microglobulin: B2Mfor (TGCTGTCTCCATGTTTGATGTATCT), B2Mrev (TCTCTGCTCCCCACCTCTAAGT). siRNA-mediated ...
QPCR primers were designed to span exon–exon junction, where possible, using Primer-BLAST (NCBI). Sequences of primers are listed in Table S2. SDS-PAGE/Western blot Cells were washed with PBS and treated with Trypsin for 5 min at 37 °C. Dislodged cells were neutralized with MEF ...
qPCR was performed using QuantiNova SYBR Green PCR Kit (QIAGEN) on a MxPro Mx3005P qPCR system (Agilent Technologies) with data analysed by Mx Pro qPCR software (Agilent Technologies). Relative expression differences were calculated using the 2−∆∆Ct method. The full list of primers ...
拷贝数变异、RPPA、Western blot、BIM敲低与qPCR MEK/ERK通路拷贝数分析:量化TCGA肿瘤中BRAF、KRAS和NF1的拷贝数变化(log2转换PDX/正常比值),定义阈值:扩增≥0.5,低水平增益≤0.25,杂合缺失≤-0.25,纯合缺失≤-0.5。PDX基因组测序(包括文库构建、靶向捕获、测序和生信分析)如先前所述[27,30,31]。DNA测序数据存放...
cDNA was amplified using SYBR Green qPCR Master Mix (ROX2 plus) (EZBioscience, Roseville, USA) on a Real-Time PCR System (Roche Applied Science, Penzberg, Germany). The target mRNA levels were normalized to those of GAPDH or β-actin. The primer sequences are listed in Supplementary Table...
real-time qPCR:反应体系及反应条件采用北京全式金生物技术有限公司(TransGen)荧光定量PCR试剂盒进行优化,确定最佳的模板浓度、退火温度,确保PCR引物的特异性及PCR扩增效率(引物见表1)。在冰上进行配制real-time qPCR扩增反应体系:2×TransStarTM Top Green qPCR SuperMix 10.0 μl;Forward Primer(10 μmol/L)0.4...
Mouse Bcl2l14 qPCR Primer Pair,即小鼠Bcl2l14 qPCR引物对,主要用于基于SYBR Green的qPCR、One-Step qRT-PCR或semi-quantitative PCR。本引物为预先设计、经过qPCR验证、预混的引物对。 qPCR (Quantitative PCR)即定量PCR,也称实时荧光定量PCR或实时定量PCR (Real-time quantitative PCR)、实时PCR (Real-time PCR...