3.主要功能是与抗原(包括外来的和自身的)相结合,从而有效地清除侵入机体内的微生物、寄生虫等异物,抗体(antibody)是一种应答抗原产生的、可与抗原特异性结合的蛋白质。 先将组织或细胞中的某种化学物质提取出来,以此作为抗原或半抗原,通过免疫动物后获得特异性的抗体,再以此抗体去探测组织或细胞中的同类的抗原物质...
Western Blotting 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. Specificity/Sensitivity BCL2L10 Antibody detects endogenous levels of total BCL2L10 protein. ...
Monoclonal Antibody for studying Bcl-2 (Ser70) phosphate. Validated for F, WB. Available in 3 sizes. Highly specific and rigorously validated in-house, Phospho-Bcl-2 (Ser70) (5H2) Rabbit Monoclonal Antibody (CST #2827) is ready to ship.
应用: Western Blot, ELISA 性状: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4 溶解方法: Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/ml in sterile distilled water by gentle pipetting 2-3 times, don’...
Rabbit anti-mouse cleaved-caspase-3 antibody (CST, Danvers, MA) was used as secondary antibody, followed by incubation with HRP-conjugated goat antirabbit IgG (Invitrogen, Carlsbad, CA). Diaminobenzidine was used to develop the staining reaction. Statistical analysis All data were analyzed with ...
Subsequently, the beads–antibody complex and protein lysate were suspended. The beads were collected by centrifugation at 3000 g, followed by three washes with lysis buffer. Following this, the immunoprecipitates were analyzed using western blot. Secondary antibodies such as anti-rabbit IgG light ...
本Bcl-2抗体(Bcl-2 antibody)为进口分装,用人工合成的Bcl-2羧基端(carboxy-terminal)的一段多肽进行适当修饰后免疫rabbit,然后用protein A和抗原多肽亲和柱经过两步纯化而得到的高纯度抗体。 本Bcl-2抗体可以识别内源性水平的Bcl-2,在生理水平检测时未发现和其它Bcl-2家族蛋白有交叉反应。 Bcl-2是一种抗凋亡蛋...
价格:￥电议 更新日期:2024/10/16 15:59:19 生产地:美洲 产品型号: 简单介绍:厦门慧嘉生物长期专业销售B0774 BCL-2 (Ab-69) Antibody \Santa\Abcam\ Cst\jackson\Pierce\Sigma\Amresco\Qiagen\Cayman \abnova\millipore\invitrogen\merk\ebioscience\prospec\细胞因子特价...
The slides were incubated with a 1:300 dilution of the anti-BCL2L10 antibody (PA5-22190, Invitrogen) for 1 h at 30 ◦C in a humidified chamber. The signal was detected using VECTASTAIN® Elite ABC Kit according to manufacturer protocol, followed by the staining with the DAB Substrate...
In analogy to the experiment performed in U87 GBM cells, LN229 and GBM22 were treated with THZ1 for 24 h, harvested, and subjected to CHIP with the same antibody against H3K27ac, but in addition, we utilized an IgG control antibody as well. Following preparation, real-time PCR analysis ...