上海玉博生物科技有限公司 主要致力于“BCA (tm) Protein Assay Kit,BCA蛋白定量试剂盒 23225 KIT”的生产销售。多年的“BCA (tm) Protein Assay Kit,BCA蛋白定量试剂盒 23225 KIT”生产与销售的经验,与各行业新老用户建立了稳定的合作关系,我公司经营的产品名称深受广大
A total of 2.5 µg of shRNA was transfected into the KGN cell line using Lipo8000™ (C0533; Beyotime Biotechnology, Shanghai, China) according to the manufacturer’s instructions. After 72 h of cell culture, 20 ng/mL BrdU (5002-1G; Sigma, St. Louis, MO, USA) was added for 2 ...
All drugs were tested in combination with 17 b-oestradiol or 4-hydroxytamoxifen (Sigma- Aldrich Chemie, Zwijndrecht, the Netherlands) or ICI182,780 (Zeneca Pharmaceuticals, Macclesfield, UK). To assay the effects of oestrogen, cells were seeded at a density of 5000 cells per well in 100 ml...
Total RNA was extracted from whole testes using Trizol reagent (Invitrogen, 15596-026) following the manufacturer's instructions. After removing the residual genomic DNA with the DNase I Kit (Promega, M6101), 500 ng of total RNA was reverse-transcribed into cDNAs using the PrimeScript RT ...
cells were stained with 10 μM C11-BODIPY581/591probe (#D3861, Invitrogen) for 30 min. Analysis of C11-BODIPY581/591fluorescence was conducted using a BD Accuri C6 flow cytometer (BD Biosciences). The concentration of GSH was detected using a Glutathione assay kit (CS0260; Sigma) by...
上海玉博生物科技有限公司 主要致力于“BCA Protein Assay Reagent (bicinchoninic acid),BCA (tm) Protein Assay Kit,BCA蛋白 Pierce 23225”的生产销售。多年的“BCA Protein Assay Reagent (bicinchoninic acid),BCA (tm) Protein Assay Kit,BCA蛋白 Pierce 23225”生产
The cells were treated with 10 nM oestrogen (Sigma, St Louis, MO, USA), 10 nM DES (Sigma), 1 mM ICI182780 (Sigma) and 1 mM tamoxifen (Sigma) for the time periods indicated in the figures and text and processed for the extraction of whole-cell lysates for western blot. Western ...
washed six times with sterilized distilled water and placed on Petri dishes containing half-strength Murashige and Skoog (½ MS) medium (Duchefa Biochemie, Haarlem, The Netherlands) supplemented with 0.5% (w/v) sucrose and solidified with 0.8% (w/v) bacteriological agar (Sigma, Burlington, MA...
International Journal of Molecular Sciences Article BCAS2 Enhances Carcinogenic Effects of Estrogen Receptor Alpha in Breast Cancer Cells Ángel Salmerón-Hernández 1,2, María Yamilet Noriega-Reyes 1,2, Albert Jordan 3, Noemi Baranda-Avila 1 and Elizabeth Langley 1,* 1 Departamento de ...