纯化的蛋白质可直接用于SDS-PAGE电泳,Western杂交等分析。 应用 RT-PCR、Northern Blot、Poly A筛选、斑点杂交等 组份 Buffer Lysis-DRP;CW1 Solution (concentrate);CW2 Solution (concentrate);CE Buffer;GT Solution (concentrate);NT Solution (concentrate);RNase-Free Water;PP Solution;PD Solution;EZ-10 DNA...
Samples were centrifuged at 13,000 rpm for 3 min and supernatants were separated by 12% SDS-PAGE, transferred to PVDF membranes (EMD Millipore, MA, IPVH00010). Membranes were blocked in solution (5% skim milk in TBST) for 1 h, washed with TBST for 3 times, then incubated with ...
for 10 min, and the supernatants were quantified and subjected to SDS-PAGE and western blotting on nitrocellulose membranes. For differential expression profiling of floating and sphere-forming blebbishields by western blotting, freshly isolated blebbishields from 40–60 Â T–75 flasks of RT4P ...
英文名称: EZ-10 Spin Column Bacterial Genomic DNA Isolation Kit 保质期: 1-2年 个月 保存条件: 常温 A. 如果 Digestion Solution 出现沉淀 , 请 55°C 水浴溶解后使用。B. 使用前加 24 ml 100% 乙醇到 8ml Wash Solution 或加 48 ml 100% 乙醇到 16ml Wash Solution,如果在运输中外漏导致Wash...
SDS-PAGE, Western Immunoblotting, and densitometry. The cells/blebbishields were washed with PBS and resuspended in lysis buffer [50 mM Tris-HCL (pH 7.4), 150 mM NaCl, 5 mM EDTA, 25 mM NaF, 1% iTnrhitiobnitoXr-c1o0c0k,t1a%il (NRoPc-4h0e,)]0a.1n md iMncNubaa...
Thus the VHL oligomers are formed possibly by covalent protein modifications, but the oligomers are held together during SDS-PAGE, independent of di-sulfide linkage(s) between VHL proteins. SDS-resistant com- plexes are formed between proteins when ATP levels go down45 and K-Ras, BAD, p27, ...
Pulldown complexes were washed four times with magnesium-containing lysis buffer before being subjected to SDS-PAGE and immunoblotting. Protein interaction database submission The protein interactions from this publication have been submitted to the International Molecular Exchange Consortium (IMEx Consortium...