BBI解决方案已经为全球诊断行业服务了50多年,作为一家开发和制造商的原材料和成品测试平台的体外诊断市场。 BBI解决方案已经为全球诊断行业服务了50多年,作为一家制造商的原材料和成品测试平台的体外诊断市场。 The BBI Group offers a portfolio of products and services that are provided to the diagnostic, healt...
纯化的蛋白质可直接用于SDS-PAGE电泳,Western杂交等分析。 应用 RT-PCR、Northern Blot、Poly A筛选、斑点杂交等 组份 Buffer Lysis-DRP;CW1 Solution (concentrate);CW2 Solution (concentrate);CE Buffer;GT Solution (concentrate);NT Solution (concentrate);RNase-Free Water;PP Solution;PD Solution;EZ-10 DNA...
Samples were centrifuged at 13,000 rpm for 3 min and supernatants were separated by 12% SDS-PAGE, transferred to PVDF membranes (EMD Millipore, MA, IPVH00010). Membranes were blocked in solution (5% skim milk in TBST) for 1 h, washed with TBST for 3 times, then incubated with ...
Pulldown complexes were washed four times with magnesium-containing lysis buffer before being subjected to SDS-PAGE and immunoblotting. Protein interaction database submission The protein interactions from this publication have been submitted to the International Molecular Exchange Consortium (IMEx Consortium...
All the blots were aligned on the basis of molecular weight. Note the oligomeric patterns of p19-VHL isoform (monomer, dimer, trimer, tetramer, and HMW VHL), RT4P, and RT4 bladder cancer cells. Suspension cells: Raji, Jurkat, HL-60, and U937. SDS-PAGE: 15% gel; 30 μg per ...
英文名称: EZ-10 Spin Column Bacterial Genomic DNA Isolation Kit 保质期: 1-2年 个月 保存条件: 常温 A. 如果 Digestion Solution 出现沉淀 , 请 55°C 水浴溶解后使用。B. 使用前加 24 ml 100% 乙醇到 8ml Wash Solution 或加 48 ml 100% 乙醇到 16ml Wash Solution,如果在运输中外漏导致Wash...
Thus the VHL oligomers are formed possibly by covalent protein modifications, but the oligomers are held together during SDS-PAGE, independent of di-sulfide linkage(s) between VHL proteins. SDS-resistant com- plexes are formed between proteins when ATP levels go down45 and K-Ras, BAD, p27, ...
for 10 min, and the supernatants were quantified and subjected to SDS-PAGE and western blotting on nitrocellulose membranes. For differential expression profiling of floating and sphere-forming blebbishields by western blotting, freshly isolated blebbishields from 40–60 Â T–75 flasks of RT4P ...