RESULTS Characterization of basophils in lymphopenic mice Lymphopenic SCID mice are deficient in T and B cells but show normal numbers of basophils (approximately 25 basophils per ml blood). We investigated whether reconstitution of SCID mice Day 7 Day 7 No T cells 20 With T cells 15 10 * ...
Splenocytes were treated with red blood cells (RBC) lysis buffer (Sigma-Aldrich, St. Louis, MO) before suspension. Bone marrow (BM) cells were flushed from the femurs and tibias. To prepare peritoneal exudate cells (PEC), the peritoneal cavity was lavaged with 10 ml of PBS using a ...
N. brasiliensis infection induced accumulation of basophils in lung, blood, and lymph nodes in 4get but not in Mcpt8Cre-4get mice. Importantly, the numbers of eosinophils and Th2 cells were not significantly different at any time in these organs in both mouse strains. We further observed ...