Functional characterization of the 40%-identical ortholog from Mycobacterium tuberculosis, a potential target for the development of novel anti-tuberculosis drugs, supports the proposed mechanism of substrate binding and catalysis. This work therefore provides a structural and functional framework to ...
a clearly visible phenylalanine (F917) in the cryo-EM structure, but a movement of the downstream region of the α-helix, possibly due to a different overall conformation of the entire structure. Furthermore, the AlphaFold2 structure recreates clearly visible cryo-EM density for almost the ...
22 In addition, a monoclonal antibody that targets complement C5a and thereby blocks its interaction with C5aR1, has recently been approved for use in hospitalized patients of COVID-19.23 However, despite its fundamental importance, our current understanding of complement anaphylatoxin recognition by...
AdvancedFeatures27 SAPGUIforHTML28 SAPGUIforJava29 FrontendinaWANEnvironment30 UnitSummary31 FurtherDocumentation32 ComputerAidedTestTool33 ComputerAidedTestTool34 CATT:Introduction35 CATT:Uses36 CATT:OtherUses37 ProcessesLessSuitedforCATT38 CATT:InitialScreen39 CATT:RecordingTransactions40 CATT:CreatingaTest...
The simulations were run on a 16-core, 64-bit SUN workstation with 64 GB of RAM. With this HPC workstation, it took less than 2 h to compute four unknowns for our model space, which are the three velocity components and the pressure gradient in the space spanned by all 2.5×108 ...
AdvancedFeatures27 SAPGUIforHTML28 SAPGUIforJava29 FrontendinaWANEnvironment30 UnitSummary31 FurtherDocumentation32 ComputerAidedTestTool33 ComputerAidedTestTool34 CATT:Introduction35 CATT:Uses36 CATT:OtherUses37 ProcessesLessSuitedforCATT38 CATT:InitialScreen39 CATT:RecordingTransactions40 CATT:CreatingaTest...
After collecting IMAC beads into a column and washing them with binding buffer, protein was batch-eluted with IMAC elution buffer (20 mM Na-HEPES pH 7.8, 150 mM NaCl, 500 mM imidazole). HEK293S cell-expressed mMBP-VR3, mMBP-VR3/lysin, VR3/lysin and VR2+/lysin were deglycosylated ...
Julie A. Nordlee5, Joseph L. Baumert5, Milos Milcic1, Steve L. Taylor5, Nuria Garrido Clua2, Tanja Cirkovic Velickovic1,* & Stef J. Koppelman5,* Conglutins represent the major peanut allergens and are renowned for their resistance to gastro- intestinal digesti...
For comparison, we also assessed the cooling distance from water bodies. From the results, it can be concluded that the cooling distance of water areas is slightly lower compared to GTl (Figure 6), but higher than the cooling distance of the other categories of green space. The average LST...