凝膠滲透層析(GPC)也稱為粒徑篩析層析(SEC),實際上是所有液相層析技術中最容易理解的一項技術。這項技術的分離機制嚴格以樣品在溶液中的大小為準據,不會像使用傳統HPLC一樣,與管柱填料產生交互作用(吸附、分配等)。分離模式並非依據分子量,而是依據分析材料(通常是聚合物)在溶液中的大小。換句話說,要正確進行GPC...
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体积分离简介 凝胶渗透色谱(GPC)也称为体积排阻色谱(SEC),其原理在所有液相色谱技术中最容易理解。分离严格基于样品在溶液中的大小,且不会像常规HPLC分析一样,与色谱柱填料发生相互作用(吸附、分配等)。分离模式并非基于分子量,而是基于所分析材料(通常是聚合物)在溶液中的大小。换言之,为正确进行GPC分析,样品必须...