Microtitration was adapted to deter mine the acidic-basic property and the change of the buffering systems in different pH of the aqueous solution in which the PM is suspended. The major alkalinity and buffer capacity of par ticles were analyzed and calculated. The average carbonate buffer ...
ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and...
Human bFGF, encoded by the FGF2 gene, is a member of the fibroblast growth factor (FGF) family. Fibroblast growth factor was found in pituitary extracts in 1973 and then tested in a bioassay that caused fibroblasts to proliferate. After further fractionating the extract using acidic and basic ...
This paper describes the production of a universal buffer system which can be used for acidic, neutral and basic mobile phases in HPLC. The mixture of alkylamine and phosphoric acid diluted with water gives the possibility to carry out separations with basic buffers on reversed phase columns with...
FGF Acidic复溶: 1、试剂开盖前请先离心。 2、复溶浓度应大于100 μg/ml. 3、冻干蛋白溶于ddH2O中 4、复溶后根据使用量分装保存,避免反复冻融。 关于FGF Acidic: FGF acidic, also known as ECGF, FGF-1and HBGF-1, is a non-glycosylated heparin binding growth factor that is expressed in the ...
Using a Becton Dickinson Cell Deposition Unit instrument (Becton Dickinson, Fullerton, CA), single cells were plated in a 96-well plate directly into 4 μL lysis buffer [50 mmol/L Tris-HCl, pH 8.3; 75 mmol/L KC1; 3 mmol/L MgCl2; 2 μmol/L of each deoxyribonucleotide triphosphate (Ph...
Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20保存建议 This lyophilized preparation is stable at 2-8...
Escherichia coli. 溶解建议 We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working...
Sperm samples were diluted in 200 μl of McIlvaine’s buffer (2 × 106sperm) and centrifuged at 300 g for 5 min; the supernatant was discarded, and the sample was resuspended in 200 μl of Carnoy solution (3:1 methanol: acetic acid) and incubated at 4 °C for 5 min. Samples...
Tissue samples obtained from the reperfused ischemic middle cerebral artery territory and homologous contralateral brain tissue were lysed in NP40 lysis buffer (50 mM Tris/HCl pH 7.5, 150 mM NaCl, 0.5% NP-40, 2 mM EDTA) containing protease inhibitor cocktail (Roche, Mannheim, Germany). Equal ...