Thus, the presence of two resistance markers allowed for dual selection during the transfection experiments. Besides testing the Drosophila-optimized Cas13 variants, we also examined the efficiency of the original Cas13 orthologs in the Sg4_CD cell line (Additional file 2: Tables S1, Additional ...
These experiments were performed as described by Schneider et al.35. Briefly, each flies’ trajectory was tracked from the videos using Ctrax (open source, versions 0.3.2 and 0.5.18) to track fly movement, orientation and identity. Ctrax-Fixerrors was used to manually inspect/correct fly traje...
such an adaptation should be similar to using multiple body parts. The challenge will most likely be the precise definition of social triggers and the design of relevant experiments using closed-loop stimulation. We briefly tested closed-loop multiple animal tracking using a pair of differently colo...
The average and relative standard deviation (% CV) for the contribution (% total) of each chain to ACE-05, determined from five independent experiments, was analyzed using 2100 Expert Software (Agilent Technology) and plotted using GraphPad Prism 8 software. The quality and conformation of ALiCE...
substrate and the transposase expression cassette, respectively, in a broad range of ratios ranging from 1:17 to 33:1 in HeLa cells (keeping the total amount of DNA constant) dramatic inhibitory effects on transposition were observed with higher doses of transposase [156]. In these experiments,...
*This sequence was the most significantly over-represented 8-mer in the upstream regions of genes that were downregulated upon overexpression of the Rox1 gene (a known repressor of hypoxia-induced genes under aerobic conditions [95]), as part of a series of microarray experiments measuring S. ...
In Fig. 7 we show the results of experiments on the influence of CeO2 nanoparticles on the life time of a fruit fly Droso phila melanogaster. From the presented data it is seen that upon introduction of CeO2 nanoparticles the life time of flies significantly increases. A fact attracting ...
(see Methods). Fig.1g plots the memory footprint as a function of the number of neurons in each tile for different network sizes. Horizontal dashed lines show the number of memory elements using one large crossbar for each network size, as has previously been used for Recurrent Neural ...
The experiments were conducted at 50 °C to maintain a steady temperature throughout the studies. Resistance was calculated from current-voltage curves swept between −0.1 to 0.1 V using Keithley 2635A instrument. The initial chamber atmosphere was air, and Pt/SNO sample was placed onto ...
In all quantitative experiments, the initial parameters of the competitor algorithms were set according to the reference papers, as shown in Table 1. Table 1. Initial parameters of the competitor algorithms. 5.2. Benchmark Test Functions The quality of optimization algorithms is commonly examined by...