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(BioRad) using the following program: 37 °C for 30 min, 95 °C for 5 min, ramp down to 25 °C at a rate of 5 °C/min. The annealed oligonucleotides were diluted 1:100 in H2O and ligated into the vector backbone using 50 ng digested lentiGuide-Puro plasmid, 1...
It has been shown that ABEs have cytosine deaminase activity in the confined TC*N sequence context, suggesting that ABE enables simultaneous A/C conversion16. Our findings also indicate that ABE8e induces simultaneous A/C conversion across four targets containing a TC*N motif, but with an editi...
Across all timeframes, the average recruitment rate was five per month, coupled with a consistent conversion rate of 97% and a remarkable 97% response rate to the questionnaires. A correlation existed between patient-related factors and clinical outcomes; the strength was fair to moderate at 12 ...
However, the conversion from the most steric hindered isomer form of sec, rac- was slow. The ratio of the isomerization rate constants between meso-CH3Co(H2O)L2+ and sec, rac-CH3Co(H2O)L2+ to pri, rac-CH3Co(H2O)L2+ is almost a factor of 100. The thermodynamic activation parameters ...
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C and 35 % relative air humidity (estimated absorption rate: 0.2 – 0.4 μg cm?2h?1), but increased by 21 and 29 % when the temperature was elevated by 5 ?Cand the humidity from 35 to 75 %, respectively . Likewise, when applied as liquid to human skin from a drained gauze (...
A Benjamini-Hochberg False Discovery Rate (FDR) was applied and 372 out of 529 RT passed FDR at 2.5%. As a result, 372 RT (19% of 1,959) showed significantly greater SBS in cancer than in healthy cells (Additional file 4). The same Ho was tested against the alternative hypothesis ...
We chose to combine replicates in this way (rather than, for example, averaging the frequencies for each replicate) because it is the maximum-likelihood estimate of the rate parameter of a hypothetical Bernoulli distribution that describes the base editing efficiency at a given position. In our ...
that APOBEC-based CBEs can stochastically deaminate single-stranded regions of the genome. We found that CBE-T1.14 catalyzed C-to-U deamination withkappof 0.060 ± 0.006 min−1, a rate indistinguishable from that measured for A-to-I deamination by ABE8.20 (Fig.5cand Supplementary...