Base editing efficiencies are reported as the percentage of sequencing reads containing a given base conversion at a specific position. Prism 10 (GraphPad) was used to generate dot plots and bar plots. Western blotting Protein was analysed via western blotting and detected using mouse anti-RPE65 ...
For each position of the 201 bases, we searched for the tetranucleotide, pentanucleotide, and hexanu- cleotide motifs and the base composition abundance at each of the 201 nucleotide positions/locations. When calculating the percentage of mRNAs that had at least one copy of the motif per mRNA...
Base editing efficiencies are reported as the percentage of sequencing reads containing a given base conversion at a specific position. Prism 10 (GraphPad) was used to generate dot plots and bar plots. Western blotting Protein was analysed via western blotting and detected using mouse anti-RPE65 ...
Base composition around poly(A) sites in comparison between NCBI mRNA-based mapping and Illumina HiSeq reads-based mapping.XiuQing, Li
(D) Percentage of cells with a detected CROP-seq transcript and with a discernable dominant sgRNA in the scRNA-seq library sequenced at an average of 20,000 reads per cell and in the enrichment PCR library, respectively. (E) Scatter plot of the total sgRNA counts per cell directly detected...
You won't be surprised to hear that a very large fraction of persistent XML is found in relational databases, right along with other data vital to an enterprise's business. Most large businesses today – and an increasing percentage of smaller businesses – depend on relational databases to sto...
aPlot representing the percentage of DNA replication origins in each quantile that overlap a promoter region (±2 Kb of TSS) of a GENCODE gene (in red). Overlaps with control regions (paler colour) which are randomly shuffled genomic regions of equal size and number as the origins are al...
The top row in the table represents the composition of the buffer encoded by symbols (in the legend); the buffers’ pKa values are marked with these symbols on the grey arrow (target pH values marked with red arrows). Some components have two pKa values making them more suitable for ...
The percentage of contigs with nominal coverage of n-fold (Y-axis) is plotted against the number of sequencing reads (X-axis). Sequencing sub-samples of a given size were randomly selected from the total pool of sequencing reads. Three replicates were performed for each data point. The mean...
Base editors enable the direct conversion of target bases without inducing double-strand breaks, showing great potential for disease modeling and gene therapy. Yet, their applicability has been constrained by the necessity for specific protospacer adjace