The external preparation containing calcium chelating taurine main body having a role in repairing epidermal barrier system, in order to protect the skin, moisturizing, firming, anti-allergic effect, etc., an external preparation can be used in cosmetics, pharmaceutical preparations for external use, ...
Dialysis is a membrane barrier separation process, in which a concentration gradient forces one or more sample analytes to transfer from one fluid to another through a membrane. From: Comprehensive Sampling and Sample Preparation, 2012 About this pageSet alert ...
GAS colonization in the pharynx is considered to be fundamental for development of these sequelae and invasive diseases, albeit pharyngeal epithelial cells function as a physical barrier to protect underlying sterile tissue. Cell-cell and cell-matrix junctional adhesions are essential for maintenance of ...
Preparation of the animals on the day of experiment was as described previously [25]. Briefly, the mice were anesthetized with ketamine (125 mg/kg), medetomidine (0.2 mg/kg), and atropine (0.5 mg/kg). A tracheotomy was performed and mechan- ical ventilation was started...
Self-reported skin discomfort is a common problem during pregnancy, but it is not clear whether skin barrier function is altered in the process. Few studies have described the skin barrier function during pregnancy. In this work, we used highly sensitive
Intestinal tissue sample preparation Mice were euthanized using pentobarbital sodium anesthesia. The intestine adjacent to the cecum was extracted and divided into upper and lower sections. The bottom section was subjected to fixation in a solution of 4% paraformaldehyde to facilitate subsequent sectioning...
The aim of this study was to determine whether and how Zn proteinate with moderate chelation strength (Zn-Prot M) can alleviate heat stress (HS)-induced intestinal barrier function damage of broilers. A completely randomized design was used for comparati
Library preparation and sequencing were carried out using similar protocols described for fecal and cecal samples (see below). Illumina Sequencing and Data Analysis PCR and library preparation were performed by the University of Michigan Microbial Systems Molecular Biology Lab as described by Kozich et ...
The human skin has a multi-layered structure composed of various cell types, fibers, lipids, and other components. It is the primary organ to protect the body from the external environment1. The skin surface maintains acidic, desiccated, and aerobic environments, whereas sebaceous follicles maintain...
RNA was extracted using TRIzol (TRI reagent, Sigma) according to the manufacturer’s guidelines. The collected samples were submitted to GENEWIZ South Plainfield, NJ, for next-generation sequencing. After quality control and RNA-seq library preparation the samples were sequenced with Illumina HiSeq ...