Plasmablasts were gated on CD20-CD38++ B-cells and further characterized as IgM+, IgA+ or IgG+. Quadrants were set based on the expression values obtained with fluorescence minus one (FMO) and isotype controls. (B) The frequencies (%) of IgM+ (left panel), IgA+ (middle panel) and ...
(a–c) Within lymphocytes, CD56+ NK cells, CD3+ CD56neg T cells, CD3+ CD56+ NK-T-like cells and CD19+ CD20+ B cells were identified (second and third panels). B cell maturational stages were distinguished based on CD24 and CD38 expression (fourth panel). The BAFF-R expression on ...
37 These cells were more than 95% CD20+ and more than 70% naive IgD+CD38– B cells and were the source of all B cells used in this study. B-cell proliferation assays Macrophages, mono-DCs, or monocytes (0.3-5 × 104) or macrophage-conditioned culture medium (MCCM) were cultured for...
We have previously shown that excess B lymphocyte Stimulator (BLyS)/BAFF in plasma and on surface of blood dendritic cells (DC) of HIV-infected progressors coincides with B-cell dysregulations and increased frequencies of “precursor” innate marginal zone (MZ)-like B-cells. In contrast, both ...