After washing, membranes were incubated with an anti-rabbit horseradish peroxidase (HRP)-linked IgG (1:5000 dilution, Jackson ImmunoResearch), an anti-mouse HRP-linked IgG (1:5000 dilution, Santa Cruz Biotechnology), or a HRP-conjugated anti-biotin (1:5000 dilution, Cell Signaling Technology) ...
After washing, membranes were incubated with an anti-rabbit horseradish peroxidase (HRP)-linked IgG (1:5000 dilution, Jackson ImmunoResearch), an anti-mouse HRP-linked IgG (1:5000 dilution, Santa Cruz Biotechnology), or a HRP-conjugated anti-biotin (1:5000 dilution, Cell Signaling Technology) ...
163 2.1. Synthesis and Manufacturing 164 Sulfolane is a non-reactive, water-soluble dipolar aprotic industrial solvent that was first 165 eSnugstianineaebirlietyd20a1n8d, 10p, a36t7e7nted by Shell Oil Company in the early 1940s [10]. The original proces5sofo2f1 166 sulfolane synthesis is...