Re- ceptor binding is followed by translocation of the colicin molecule through the cell envelope by either the Ton or the Tol sys- tem (11, 12) The Tol-PAL system of Escherichia coli is composed of six proteins localized in the cell envelope (TolQ, TolR, TolA, TolB, PAL, and Orf2...
Sequencing (Miseq, Illumina, San Diego, CA, USA) and data processing were performed using BLASTn against a curated database from RDPII and NCBI (MR DNA, Shallowater, TX, USA). In order to analyze the bacterial diversity of the cultivable bacteria, the 16S rDNA sequences were clustered ...
4.3.2. Screening of Pathogens DNA by Standard PCR The primers were used targeting 969 bp of the conserved region of encoding ribosomal 18S and 500 bp of 16S RNA genes to detect Piroplasma [2] and Anaplasmataceae DNA [59], respectively (Table 5). PCR assays were performed using One PCR...
identify them on SS3 by TEM microscopy, Figure 3, while all of the appendages could be clearly seen by SEM microscopy (Figure S1). The fimbriae 1 in SS3, Figure 3b–d, are in the range of 7.0 nm diameter as stated in other works [31–33]. The presence of fimbriae 1 on the E....
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