A bacterial/yeast merged two-hybrid system: protocolfor bacterial screening. Serebriiskii I G,Milech N,Golemis E A. Methods in Molecular Biology . 2007I.G. Serebriiskii, N. Milech, E.A. Golemis, A bacterial/yeas
Testing protein interactions using two-hybrid systems Bacterial two-hybrid system (BACTH) analysis was performed as described before97. Gsp genes were amplified with specific primers (listed in Supplementary Data 6) and cloned into pKT25 and pUT18c plasmids. E. coli strain DHT1 competent cells ...
BACTH, Bacterial Adenylate Cyclase-based Two-Hybrid. Although the BACTH assays suggest the existence of the interaction of LplT and Aas, it is also possible that their interaction is mediated by other endogenous components in the E. coli membrane. To exclude this possibility, the interaction of...
Smart hybrid materials by conjugation of responsive polymers to biomacromolecules. Nat. Mater. 14, 143 (2014). PubMed Google Scholar Schreiber, C. L. & Smith, B. D. Molecular conjugation using non-covalent click chemistry. Nat. Rev. Chem. 3, 393–400 (2019). CAS PubMed PubMed Central...
To reveal the interactions in the VP371-GroEL-AST complex, the bacterial two-hybrid system was conducted. Only proteins that interacted with each other could induce growth of the reporter strain in LB-CTCK medium (Figure 1C). The results presented that protein–protein interactions existed ...
The interaction of AtoSC with FlhDC through yeast two-hybrid system is shown on D. E, shows the schematic representation of eGFP reporter assay. F, to check the role of MinD and AtoSC in regulating expression of fliA promoter, eGFP expression was monitored with an anti-GFP antibody. The ...
Synthesis of nanostructured lipid carriers loaded chitosan/carbopol hybrid nanocomposite gel for oral delivery of artemether and curcumin. Pharm Nanotechnol. 2020;8(5):418–32. Article CAS PubMed Google Scholar Bueloni B, et al. Design of nalidixic acid-vanadium complex loaded into chitosan hybrid...
(Figure2c-f). In the two-step treatment of Series 2 with higher microalgae concentration, the ethanol concentration remained at 51–60 mg carbon per liter in the first step until granular sludge was added (Figure2h). The levels of total VFAs also increased from 185 to 457 mg carbon per ...
(SGF) is achieved with structural integrity and bioactivity in hydrogel forms. BC is mixed with octenidin:poloxamer hybrid system to form a membrane and achieve a long-term controlled release of octenidine to assist in the advanced treatment of infected wounds with improved mechanical and ...
A modified protocol of the adenylate cyclase-based bacterial two-hybrid technique was used36. Briefly, the isolated T18 and T25 catalytic domains of the Bordetella adenylate cyclase were fused to the N and C-termini of the proteins to be tested. After transformation of the two plasmids producin...