DNA purity and concentration were measured using a NanoDrop spectrophotometer. The minimum quality (ng/µL) and purity values (ratio 260/280) accepted for qPCR analysis were 5 and 1.5, respectively. Genomic DNA was isolated from peripheral blood with Gentra Puregene Blood Kit (Qiagen), as ...
Methods and Results Human milk DNA was extracted using the following methods: (i) Qiagen MagAttract Microbial DNA Isolation Kit (kit QM), (ii) Norgen Milk Bacterial DNA Isolation Kit (kit NM), (iii) Qiagen MagAttract Microbiome DNA/RNA Isolation Kit (kit MM) and (iv) TRIzol LS Reagent (...
The molecular-based identification was performed, using 16S rRNA sequencing. Thermo Gene JET Genomic DNA Purification Kit (#K0721) was used to extract the bacterial genomic DNA. The 16S rRNA gene was amplified by PCR following the protocol of El-Naggar et al.43. The Qiaquick spin-gel extract...
The processing of MBG water through the MoBio PowerSoil DNA Isolation Kit typically yielded an optical density (OD) reading at 260 nm suggesting 19–25 ng DNA per µl. The 260/280 OD ratio was, however, always >2.0 and produced a flattened spectral peak suggesting the presence of ...
The processing of MBG water through the MoBio PowerSoil DNA Isolation Kit typically yielded an optical density (OD) reading at 260 nm suggesting 19–25 ng DNA per µl. The 260/280 OD ratio was, however, always >2.0 and produced a flattened spectral peak suggesting the presence of ...
Although the human bladder is reported to harbor unique microbiota, our understanding of how these microbial communities interact with their human hosts is limited, mostly owing to the lack of isolates to test mechanistic hypotheses. Niche-specific bacte
RNA was isolated with the Agilent Total RNA Isolation Mini Kit following the detailed protocol in Appendix A, or by a silica-based method (QIAGEN RNeasy and RNase-free DNase Set, QIAGEN, Valencia, CA). RNA quality was determined via the Prokaryotic Total RNA Nano asasy from the RNA 6000 ...
The main methods investigated were as follows; Qiagen QIAmp Blood kit, Roche high PCR template preparation kit, Puregene DNA extraction kit, boiling, glass beads/sonication and wash/alkali/heat lysis. The results indicated that a simple wash/alkali/heat lysis method was the most sensitive, ...
The genomic DNA was extracted from 0.30 g of soil using the MoBio PowerSoil DNA Isolation Kit (QIAGEN, Ins., USA). The V3-V4 regions of the bacterial 16S rRNA gene were amplified using universal primers 338F (5’-ACTCCTACGGGAGGCAGCAG-3’) and 806R (5’-GGACTACHVGGGTWTCTAAT-3’)...
. The total RNA was prepared using the RNA Extraction Kit (QIAGEN, Germany) and rRNA was removed using Ribo-Zero rRNA Removal Kit (Epicentre, United States), following the manufacturer’s protocol. Sequencing libraries were generated using the NEBNext Ultra Directional RNA Library Prep Kit for ...