Cell Culture TechniquesSolutionsBasic information for the culture of bacteria is presented in this appendix, using Escherichia coli as a representative organism. The techniques and recipes should be readily transferrable. Specifics are given for liquid and solid media, rich and minimal media, stab ...
chapter, we discuss the procedures and protocols used to describe the mechanism of action of the kinase KinC as a membrane kinase that senses potassium leakage from the cytoplasm of the cell. We analyze the methods and techniques used, highlighting tricks discovered over the course of the study...
Such techniques are known for their selectivity and are not considered representative of the extent of the bacterial community. The proportion of cells which can be cultured is estimated to be 0.1% or at most 10% of the total population [1] and few data are available concerning how closely ...
Bacterial genetics articles from across Nature Portfolio Atom RSS Feed Bacterial genetics is the study of the mechanisms of heritable information in bacteria, their chromosomes, plasmids, transposons and phages. Techniques that have enabled this discipline are culture in defined media, replica plating,...
The current culture-based approach for the diagnosis of bloodstreams infection is incommensurate with timely treatment and curbing the prevalence of multi-drug resistant organisms (MDROs) due to its long time-to-result. Bloodstream infections typically involve extremely low (e.g., <10 colony-forming...
Agents such as polyethylene glycol (PEG) have also been shown to induce bacterial cell transformation (7). We now describe a new method for the preparation of competent bacterial cells that yields high transformation efficiencies but is simpler and more convenient than other techniques. Bacterial ...
Bacteroid proteins were obtained by sonication at 24 kHz 1 min ON/1 min OFF for 5 cycles at 4°C in a Vibra Cell (Sonics, USA) in the presence of a protease inhibitor (Complete tablets, Roche Diagnostics GmbH, Mannheim, Germany). To further limit proteolysis, protein isolation was ...
The LIVE/DEADBacLight Bacterial Viability Kits are convenient and easy-to-use kits for monitoring the viability of bacterial populations based on the membrane integrity of the cell. These kits provide a two-color fluorescence assay of bacterial viability for a diverse array of bacterial genera. Ce...
Currently, defining a cell’s state most frequently involves measuring its transcriptional profile. Accordingly, numerous techniques have been developed that can determine the identity and abundance of RNA transcript levels as well as whether the abundances are actively changing8,9,10. Measurements can ...
However, the collection of suitable samples for culture may face limitations due to the organism's limited viability, which requires immediate freezing for preservation. Genotypic analysis is also constrained by limitations in bacterial cultivation, but recent advancements in DNA enrichment techniques have...