The log phase is marked by rapid exponential growth. The rate at which cells grow during this phase is known as the growth rate (k), and the time it takes the cells to double is known as the generation time (g). During log phase, nutrients are metabolised at maximum speed until one...
The invention generally relates to compositions and methods for isolating mid-log phase bacteria. In certain embodiments, the invention provides a magnetic particle conjugated to an antibody that includes an epitope specific for mid-log phase bacteria....
Bacterial flagella were isolated, depolymerized, and repolymerized as previously reported24,38,44. First, a log-phase culture ofSalmonella typhimurium(SJW1103) was obtained by an overnight culture of the bacteria in a modified LB broth (1% tryptone(w/v), 1% yeast extract(w/v), 0.1% glucose...
The culture was then diluted 1:100 into fresh TSB and incubated again for 4 h 20 min at 37 °C while shaking at 150 rpm until the culture reached its mid-log phase at a point where the bacteria were experimentally found to be most motile (OD600 ~1.4). Subsequently, 0.1...
Background: Bacterial contamination of blood components is primarily attributed to the phlebotomy process during which bacteria, likely in a stationary rather than log growth phase, gain entry into the donor unit. Since bacteria in their stationary phase may be particularly difficult to eradicate, we...
1 PhysiologyoftheBacteria BacterialchemicalcomponentsandPhysicalcharacterGrowthofBacteria Growthrequirements BacteriaMetabolismCultivationofBacteria 2 1.1Bacterialchemicalcomponents Water:75%-90% Inorganicsalt Protein:50%-80%ofdryweightaccordingbacterialkindsandage.Sugar:mainly...
Sequential fermentation increases viable counts ofLactobacillusin apple juice.Sequential fermentation allowsLactobacillusto exhibit two log phases.Sequential fermentation promotes biotransformation in apple juice.Fermented apple juice markedly enhances the antioxidant activity.Fermented apple juice preserves and generat...
Antifungal endophytic bacteria were cultured in TY agar medium at 30 °C to the mid-log phase.47 Cells were pelleted by centrifugation (3440 × g, 10 min at 4 °C), washed twice with a sterile saline solution, and prepared for bacterial suspensions (approximately 108 cfu mL−1). The ...
log-phase of growth and involves the oversynthesis of specific groups of proteins designed to protect the cell to specific stress condition; (2) general systems, where the adaptation to one stress condition can render cells resistant to other stress; and (3) stationary-phase associated stress ...
Jobin M-P, Garmyn D, Divies C& Guzzo J (1999) TheOenococcus oeniclpX homologue is a heat shock gene preferentially expressed in exponential growth phase. J. Bacteriol. 181: 6634–6641. Google Scholar Johnson JL, Phelps CF, Cummins CS, London J& Gasser F (1980) Taxonomy of theLactobaci...