The log phase is marked by rapid exponential growth. The rate at which cells grow during this phase is known as the growth rate (k), and the time it takes the cells to double is known as the generation time (g). During log phase, nutrients are metabolised at maximum speed until one...
The invention generally relates to compositions and methods for isolating mid-log phase bacteria. In certain embodiments, the invention provides a magnetic particle conjugated to an antibody that includes an epitope specific for mid-log phase bacteria....
Bacterial flagella were isolated, depolymerized, and repolymerized as previously reported24,38,44. First, a log-phase culture ofSalmonella typhimurium(SJW1103) was obtained by an overnight culture of the bacteria in a modified LB broth (1% tryptone(w/v), 1% yeast extract(w/v), 0.1% glucose...
The cultures were allowed to grow at 37 °C until the mid-log phase. Protein production was induced with 0.2% w/v L-arabinose with incubation at 30 °C for ~17 h. Cells were harvested by centrifugation at 8000 × g for 5 min, resuspended in lysis buffer (0.01 M MOPS)...
Sequential fermentation increases viable counts ofLactobacillusin apple juice.Sequential fermentation allowsLactobacillusto exhibit two log phases.Sequential fermentation promotes biotransformation in apple juice.Fermented apple juice markedly enhances the antioxidant activity.Fermented apple juice preserves and generat...
1 PhysiologyoftheBacteria BacterialchemicalcomponentsandPhysicalcharacterGrowthofBacteria Growthrequirements BacteriaMetabolismCultivationofBacteria 2 1.1Bacterialchemicalcomponents Water:75%-90% Inorganicsalt Protein:50%-80%ofdryweightaccordingbacterialkindsandage.Sugar:mainly...
Antifungal endophytic bacteria were cultured in TY agar medium at 30 °C to the mid-log phase.47 Cells were pelleted by centrifugation (3440 × g, 10 min at 4 °C), washed twice with a sterile saline solution, and prepared for bacterial suspensions (approximately 108 cfu mL−1). The ...
log-phase of growth and involves the oversynthesis of specific groups of proteins designed to protect the cell to specific stress condition; (2) general systems, where the adaptation to one stress condition can render cells resistant to other stress; and (3) stationary-phase associated stress ...
per hour during their log phase respectively (Additional file1: Fig. S5). Discussion Arsenic contamination in soil and groundwater in Bangladesh surpasses any incident seen before. Irrigation water in Bangladesh containing high level of As which may result in food chain contamination and loss of cr...
Jobin M-P, Garmyn D, Divies C& Guzzo J (1999) TheOenococcus oeniclpX homologue is a heat shock gene preferentially expressed in exponential growth phase. J. Bacteriol. 181: 6634–6641. Google Scholar Johnson JL, Phelps CF, Cummins CS, London J& Gasser F (1980) Taxonomy of theLactobaci...