By means of a modified Gram stain he has been able to demonstrate two varieties, or rather forms, of the tubercle bacillus which are not acid-fast. These forms he has conclusively identified both by inoculation and cultural studies and has shown that one form may develop into, or generate,...
megaterium G18 and BM13A morphology in presence of l-proline (Pro) and l-glutamate (Glu). The B. megaterium G18 and BM13A cells (6 logCFU/mL) were grown in minimal media in acid and salt stressed condition for 1 h, harvested by centrifugation at 6000 rpm for 5 min, gram stained ...
Due to the significant antagonistic activity of Bac 3 isolate it was chosen for further experimentations. The Gram stain was carried out to detect the shape, arrangement, and type of bacterial cells. As indicated in Fig.2a, this bacterium is gram positive, rod shape, and arranged as mono b...
Cell morphology can be observed at the end of simple staining. 7, mordant: add 1 drops of iodine solution, 1min dyeing, washing. 8, decolorization: suction residual water, continuous drops of 95% ethanol decolorization, 20-30s to the outflow of liquid, no purple, immediately washed. 9, ...
To get information regarding morphology, bacterial colonies have been stained with the standard Gram-stain and investigated under an oil immersion high power light microscopy (100×magnification lens). Bacterial isolate was then characterized using standard biochemical tests (ex: (Indole, VP, citrate te...
Gastrointestinal infection is a leading cause of gut diseases attracting global health concerns. The emerging antimicrobial resistance in enteric pathogens drives the search of viable and renewable alternatives to antibiotics for the health of both human
morphology of spore: Grp. 1: Gram-+ central/terminal, ellipsoidal/cylindrical spores don't distend sporangium (B. anthracis/cereus/thuringiensis) Grp. 2: Gram-variable ellipsoidal/ swollen spores B. subtilus/ coagulans) Grp. 3: Gram-variable ...
The strains identification are included the spore morphology, Gram stain, and motility. The morphological and physiological characteristics of the bacterial isolates were compared with the data from Bergey’s Manual of Determinative Bacteriology [17]. 2.5. Scanning of Bacillus sp. MA20 and Bacillus sp...
Sixty-six isolates of Gram-positive bacilli, with morphology of being large, dry, white colored, with wavy, lobed margins were obtained. After the antimicrobial activity against B. pseudomallei was observed by the agar well diffusion method, the two isolates named N2-4 and N3-8 showed clear ...
Microscope Stereo Olympus (America) was used to observe the morphology of bacterial colonies grown on nutrient agar. The growth characteristics such as structure, shape, color, margin, surface characteristics, elevation, cell's arrangement, and Gram staining of the bacterial colonies were observed to...