淀粉液化芽孢杆菌Bacillusamyloliquefaciens是一种嗜热细菌,其产生的中性植酸酶具有很好的应用前景.通过TD-PCR技术将Bacillusamyloliquefaciens编码的中性植酸酶基因克隆至原核表达栽体pET-30a(+)上,在大肠杆菌BL21(DE3)中得到了高效表达,表达量占大肠杆菌可溶性蛋白的33.5%.采用金属Ni^2+亲和层析对基因表...
Bacillus amyloliquefaeiens a new discovm~ and new applications,gene function and molecular research methodsofexploration,fermentation conditionsand medium formulation ofthe research in recentyears. Keywords Bacillus amyloliquefaciens; new discovmT; new applications; gene function; molecular research methods;...
ResearchonDevelopmentofBacillusamylotiquefaciensCheXiaoxiLiXiaokunAbstractBacillusamyloliquefacienshadbeenresearchedandexploredmoreandmorewidelywiththeabilitytoinhibitfungiandbacteriatherebyitbecomingapotentialmicrobialbio—pesticidesinrecentyears.ArticleswassummarizedontheBacillusamyloliquefaeiensanewdiscovm~andneunctionand...
(Bacillusamylolique~ciens)的研究与探索越来越多,由于其自身具有广泛地抑制真菌与细菌的能力,,基因功能与分子研究方法的探索,;新发现;新应用;基因功能;分子研究方法;发酵条件:发酵培养基培养中图分类号S182文献标志码BTheResearchonDevelopmentofBacillusamylotiquefaciensCheXiaoxiLiXiaokunAbstractBacillusamyloliquefaciens...
Bacillus amylolique faciens苹果青霉病生物防治本文主要研究了不同贮藏温度下植物内生菌Bacillus amyloliquefaciens ES-2菌株对苹果青霉病的抑制效果.ES-2菌株的各处理液在苹果果实和PDA培养基上对苹果青霉病菌均有抑制作用.较低的贮藏温度有利于拮抗菌对病菌的抑制效果;24h后接种病菌孢子的果实其病斑直径一般都高于48...
摘要近年对解淀粉芽孢杆菌(Bacillusamylolique~ciens)的研究与探索越来越多,由 于其自身具有广泛地抑制真菌 与细菌的能力,因而成为具有生物农药开发潜力的微生物.文章对近几年关于解淀 粉芽孢杆菌的新发现与新应用,基因 功能与分子研究方法的探索,发酵条件与培养基配方的研究进行了总结. 关键词解淀粉芽孢杆菌;新发现...
Author links open overlay panelLuan S J *, Sun Y B *, Wang Y *, Sa R N *, Zhang H F *Show more Add to Mendeley Share Cite https://doi.org/10.3382/ps/pey478Get rights and content Under a Creative Commons license open accessABSTRACT Studies have shown that supplementing probiotics ...
本文主要研究了不同贮藏温度下植物内生菌Bacillus amyloliquefaciens ES-2菌株对苹果青霉病的抑制效果.ES-2菌株的各处理液在苹果果实和PDA培养基上对苹果青霉病菌均有抑制作用.较低的贮藏温度有利于拮抗菌对病菌的抑制效果;24h后接种病菌孢子的果实其病斑直径一般都高于48h后接种的果实.实验证明,ES-2菌株防治苹果青霉...
Among the amino acid residues surrounding the substrate binding site, Val543 and Glu545 of BspAG31A were different from the corresponding residues of Bacillus thermoamylolique- faciens a-glucosidase II, which has higher activity toward isomaltose than BspAG31A. The E545G mu- tation slightly ...
A study on the α-amylase fermentation performed b Bacillus amylolique3faciensBacillus amyloliquefaciensα-amylaseSubstrateα-Amylase fermentation with soluble starch as the limiting substrate was performed by Both the initial and average rates for the hydrolysis influence, of the presence of α-amylase...